KLI

국내 자생 소나무류(소나무, 곰솔, 리기다소나무)의 꽃가루 항원성 연구

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Abstract
Over the years, pine pollens have been excluded as an allergen due to its relatively large size, low protein content, and waxy hydrophobic layer, despite their abundance. However, recent studies suggest the possibilities of pine pollens being allergens, and it has been reported that allergy symptoms were highly prevalent in areas with considerably large pine forests and high possibility of exposure to the pollen. Therefore, we conducted a comparative analysis of the allergenicities of the pollens from the dominant species of Korean pines, red pine (Pinus densiflora), black pine (Pinus thunbergii), and pitch pine (Pinus rigida) In order to determine specific allergens , we clarified the protein composition of various pollens which are potentially allergenic, and three different protein compositions, originated from P. densiflora, P.thunbergii, and P. rigida pollen, were identified. Next, we tested antigenicity of the pollens by sensitizing Balb/c mice. As duration of sensitization took longer via skin and airway, all three pollens were able to induce IgE increment. Notably, sensitization through airway induced more IgE than that of skin. Ability of IgE induction was superior in pollen from pine tree than two counterparts, which suggested that the pollen from pine tree is the utmost inducible factor for IgE compare to other pollens from P.thunbergii, and P. rigida. To assess whether the pollens might have antigenicity to human, we carefully measured serum IgE level of patients with pine tree pollens, and concluded that domestic pine tree originated pollens have actual allergenic effects. Analysis of IgE immunoblotting bands revealed not only common antigens (28,36,42,55,72,85kDa) which are already reported, but also new antigens from domestic pine tree originated pollens 36~42kDa, 42~55kDa, and 55~72kDa.
Author(s)
김서융
Issued Date
2018
Awarded Date
2018-02
Type
Dissertation
Keyword
PollinosisAllergenIgERed pineBlack pinePitch pine
URI
https://oak.ulsan.ac.kr/handle/2021.oak/6526
http://ulsan.dcollection.net/common/orgView/200000011290
Alternative Author(s)
Kim, Seo-Yoong
Affiliation
울산대학교
Department
일반대학원 생명과학과
Advisor
최기룡
Degree
Doctor
Publisher
울산대학교 일반대학원 생명과학과
Language
kor
Rights
울산대학교 논문은 저작권에 의해 보호받습니다.
Appears in Collections:
Life Science > 2. Theses (Ph.D)
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