KLI

전사체 분석 기반 크론병 특이 장간막 지방 조직 비후의 역할 분석

Metadata Downloads
Alternative Title
Transcriptomic analysis reveals the function of Creeping fat in Crohn’s disease
Abstract
One of the pathologic features of Crohn’s disease (CD) is a “creeping fat” (CF), mesenteric adipose tissue (MAT) wrapping the inflamed intestine. This study aims to define distinct transcriptional profile and identify cellular composition of CD-CF. We collected 59 MAT samples: 23 paired samples from CD patients with CF (CD-CF) and MAT around the uninflamed intestine (CD-MAT) and 13 MAT samples from non-CD patients (Con-MAT). Differentially expressed gene (DEG) analysis performed by DESeq2, cell deconvolution analysis performed by CIBERSORTx. We identified a total of 529 DEGs (|log2FoldChange|>1.5; false discovery rate <0.05). Of these, 323 genes showed an incremental pattern from Con-MAT to CD-MAT, and to CD-CF, while 105 genes showed a decremental pattern. Incremental pattern genes were related to immune cell responses, including B-cell and T-cell activation, while genes with a decremental pattern were involved in cell trafficking and migration. Cell deconvolution analysis revealed significant changes in cellular composition between the CD-CF and Con-MAT groups, with increased proportions of B-cells/plasma Cells (P-value=6.00E-08) and decreased proportions of preadipocytes (P-value=6.20E-04) in the CD-CF group. To conclude, dynamic transcriptional changes and altered cellular components may be crucial for the shift from Con-MAT to CD-MAT and to CD-CF, providing insight into the mechanisms behind CF and highlighting its possible role in CD pathogenesis.
Author(s)
박소정
Issued Date
2024
Awarded Date
2024-02
Type
Dissertation
URI
https://oak.ulsan.ac.kr/handle/2021.oak/12998
http://ulsan.dcollection.net/common/orgView/200000732860
Affiliation
울산대학교
Department
일반대학원 의과학과 의과학전공
Advisor
이호수
Degree
Master
Publisher
울산대학교 일반대학원 의과학과 의과학전공
Language
eng
Rights
울산대학교 논문은 저작권에 의해 보호받습니다.
Appears in Collections:
Medical Science > 1. Theses (Master)
Authorize & License
  • Authorize공개
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.