Anti-melanogenic effect of Bay 11-7082 on post-inflammatory hyperpigmentation

Abstract

Post-inflammatory hyperpigmentation (PIH) refers to an acquired pigmentation disorder that causes pigmentation due to inflammation of the skin due to external causes such as injury or skin disease. It is a serious problem that affects skin health and appearance. Various treatments have been developed to deal with this problem, but there is still no complete cure. Additionally, there is a lack of understanding of the occurrence mechanism and underlying physiological causal relationship of PIH. The molecular understanding of how inflammation affects melanogenesis and how melanin production changes after inflammation is still unknown territory. Therefore, by analyzing the mechanism of PIH, we aim to understand the interaction between inflammation and melanin production and develop an effective treatment that simultaneously controls inflammation and melanin production. UVB is one of the ultraviolet rays emitted from sunlight and activates the MAPK pathway, promoting MITF and its subordinate genes Tyrosinase, Trp1, and DCT, which are involved in melanin production. This study confirmed that Bay 11-7082 inhibits melanin increased by Forskolin (FSK) or UVB in melanocytes, mouse skin, and ex vivo human skin. Bay 11-7082 inhibits cellular tyrosinase activity increased by FSK and MAPK, it was confirmed that the levels of MITF, Tyrosinase, DCT, and Trp1 were reduced. In addition, it suppressed the levels of cytokines IL-1a, IL-1b, IL-6, TNF-a, COX-2, and MCP-1, and induced inflammation in mice with DNFB, causing pigmentation and inflammation and inflammation by Bay 11-7082. It was confirmed that melanin was reduced. As a result, Bay 11-7082 is an NF-κB inhibitor that reduces inflammation by suppressing cytokines and suppresses melanin through the MAPK/MITF pathway, so it can simultaneously suppress inflammation and melanin in the development of a treatment for PIH. It is judged to have great potential as a functional raw material. (Keywords) Post-inflammatory hyperpigmentation (PIH), Bay 11-7082, MAPK/MITF pathway, melanogenesis.