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새로운 세포외 소포체 추출 시스템을 이용한 세포외 소포체 순환 RNA 분석과 대장암의 진단

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Alternative Title
Diagnosing colorectal cancer with extracellular vesicle derived circulatory RNA analysis using a novel extracellular vesicle isolation system
Abstract
Purpose: Early detection for colorectal cancer (CRC) is challenging and crucial for effective intervention, as the 5-year survival rate declines sharply in advanced stages. Recently, liquid biopsy including small extracellular vesicles (sEVs) have gained significance in CRC diagnosis. The aim of this study was to apply a novel sEV isolation system to analyze circulatory RNA expression in CRC patients compared to healthy population.
Methods: Blood plasma samples of 80 colorectal cancer patients and 20 healthy controls were obtained from the Biological Resource Center of Asan Medical Center in Seoul, Korea. The enrolled subjects included individuals with histologically confirmed stage 0-1 (n = 20), stage 2 (n = 20), stage 3 (n = 20), and stage 4 colorectal cancer (n = 20) with healthy controls (n = 20). Integrated tool for EV isolation, EV-derived protein and EV-derived nucleic acid (NA) extraction with EV enrichment (SF-ZAHVIS) was used to isolate sEVs from plasma samples. sEV derived microRNAs (miRNAs) and circular RNAs (circRNAs) were compared between healthy control and according to each cancer stage. The efficacy of the sEV isolation system was assessed through a comparison with conventional sEV isolation techniques (ultracentrifugation [UC] and total exosome isolation [TEI] methods).
Results: The SF-ZAHVIS demonstrated an effective approach for sEV isolation, yielding purity and concentration comparable to the established UC and TEI techniques. The SF-ZAHVIS system successfully extracted EV derived non-coding RNAs and detected potential CRC miRNA markers (miR-23a-3p, miR-92a-3p, miR-125a-3p, miR-150-5p). The relative expression level of miR-23a-3p, miR-92a-3p, and miR-125a-3p was significantly higher in the CRC samples compared to healthy control (P = 0.0014, 0.0002, and 0.0274, respectively). Expression level of miR-150-5p was significantly lower in the CRC group compared to healthy control (P < 0.0001).
Conclusion: The SF-ZAHVIS system is capable of isolating sEVs efficiently. By using this novel system, miRNAs could be assessed from blood plasma to detect CRC.
Author(s)
김영일
Issued Date
2024
Awarded Date
2024-08
Type
Dissertation
Keyword
Colorectal NeoplasmExtracellular Vesicle
URI
https://oak.ulsan.ac.kr/handle/2021.oak/13216
http://ulsan.dcollection.net/common/orgView/200000805518
Affiliation
울산대학교
Department
일반대학원 의학과
Advisor
임석병
Degree
Doctor
Publisher
울산대학교 일반대학원 의학과
Language
kor
Rights
울산대학교 논문은 저작권에 의해 보호받습니다.
Appears in Collections:
Medicine > 2. Theses (Ph.D)
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