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Image-guided stent-directed irreversible electroporation for circumferential ablation in the rat esophagus

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Abstract
Background: Irreversible electroporation (IRE) has been investigated in the alimentary tract; however, the lack of dedicated electrodes and insufficient tissue responses made its application limited. The aim of this study was to investigate the efficacy and safety of image-guided stent-directed IRE in the rat esophagus.
Methods: The bipolar self-expandable electrode (SE) was developed using the braiding technique. A finite element analysis was performed to validate optimal electrical field strength for the rat esophagus. A total of 40 out of 50 rats received stent-directed IRE and were sacrificed at 10 h, 3 days, 7 days, and 28 days of 10 each. The remaining ten rats underwent a sham procedure. The outcomes of stent-directed IRE were assessed by esophagography and histological responses.
Results: Stent-directed IRE was technically successful in all rats with mild muscle contraction. The heart rate dropped immediately and gradually recovered at 180 s. TUNEL and caspase-3 with submucosal thickness significantly increased at 10 h and Day 3 compared with those of the sham control (all p < 0.001). The thickness of epithelial layers with collagen deposition significantly decreased at 10 h and Day 3 (all p < 0.001), however, increased at Day 7 compared with that of the sham control (all p < 0.05). The Ki67-positive deposition significantly increased at Day 3 and 7 compared with that of the sham control (all p < 0.001). All variables were similar to those of the sham control at Day 28.
Conclusion: Image-guided stent-directed IRE was effective and safe in the rat esophagus. It seems to have effectively and evenly induced cell death and gradually recovered with cellular regeneration.
Author(s)
Song Hee KimSeung JeongJeon Min KangYubeen ParkDae Sung RyuDong-Sung WonJi Won KimChu Hui ZengHyung-Sik KimHong Bae KimSang Soo LeeJung-Hoon Park
Issued Date
2022
Type
Article
Keyword
ablationelectrodeesophagusirreversible electroporationself-expandable stent
DOI
10.3389/fbioe.2022.934858
URI
https://oak.ulsan.ac.kr/handle/2021.oak/15584
Publisher
Frontiers in Bioengineering and Biotechnology
Language
영어
ISSN
2296-4185
Citation Volume
17
Citation Number
11
Citation Start Page
1
Citation End Page
12
Appears in Collections:
Medicine > Nursing
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