Image-guided stent-directed irreversible electroporation for circumferential ablation in the rat esophagus
- Abstract
- Background: Irreversible electroporation (IRE) has been investigated in the alimentary tract; however, the lack of dedicated electrodes and insufficient tissue responses made its application limited. The aim of this study was to investigate the efficacy and safety of image-guided stent-directed IRE in the rat esophagus.
Methods: The bipolar self-expandable electrode (SE) was developed using the braiding technique. A finite element analysis was performed to validate optimal electrical field strength for the rat esophagus. A total of 40 out of 50 rats received stent-directed IRE and were sacrificed at 10 h, 3 days, 7 days, and 28 days of 10 each. The remaining ten rats underwent a sham procedure. The outcomes of stent-directed IRE were assessed by esophagography and histological responses.
Results: Stent-directed IRE was technically successful in all rats with mild muscle contraction. The heart rate dropped immediately and gradually recovered at 180 s. TUNEL and caspase-3 with submucosal thickness significantly increased at 10 h and Day 3 compared with those of the sham control (all p < 0.001). The thickness of epithelial layers with collagen deposition significantly decreased at 10 h and Day 3 (all p < 0.001), however, increased at Day 7 compared with that of the sham control (all p < 0.05). The Ki67-positive deposition significantly increased at Day 3 and 7 compared with that of the sham control (all p < 0.001). All variables were similar to those of the sham control at Day 28.
Conclusion: Image-guided stent-directed IRE was effective and safe in the rat esophagus. It seems to have effectively and evenly induced cell death and gradually recovered with cellular regeneration.
- Author(s)
- Song Hee Kim; Seung Jeong; Jeon Min Kang; Yubeen Park; Dae Sung Ryu; Dong-Sung Won; Ji Won Kim; Chu Hui Zeng; Hyung-Sik Kim; Hong Bae Kim; Sang Soo Lee; Jung-Hoon Park
- Issued Date
- 2022
- Type
- Article
- Keyword
- ablation; electrode; esophagus; irreversible electroporation; self-expandable stent
- DOI
- 10.3389/fbioe.2022.934858
- URI
- https://oak.ulsan.ac.kr/handle/2021.oak/15584
- Publisher
- Frontiers in Bioengineering and Biotechnology
- Language
- 영어
- ISSN
- 2296-4185
- Citation Volume
- 17
- Citation Number
- 11
- Citation Start Page
- 1
- Citation End Page
- 12
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- 공개 및 라이선스
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