Epithelial-Mesenchymal Transition Phenotype and Peritumoral Immune Cell Infiltration in Advanced Biliary Tract Cancer
- Abstract
- Background/aim: This study evaluated the clinical implications of epithelial-mesenchymal transition (EMT) markers and peritumoral immune cell infiltration in patients with biliary tract cancer (BTC) treated with gemcitabine plus cisplatin (GemCis).
Materials and methods: Forty-five patients with advanced BTC who received GemCis were included as the study population. We conducted multiplex immunohistochemistry and examined EMT markers and their correlations with immune cell infiltrate at the invasive tumor margin. Study population was subdivided into two groups: twenty-four patients with overall survival (OS) less than 10 months (short-term survivor group, SS) and 21 with OS of 20 months or longer (long-term survivor group, LS).
Results: The density of tumor cells expressing epithelial marker E-cadherin (E-cadherin+ CK+) at the invasive tumor margin tended to be higher in the LS group than that in the SS group (p=0.065). The density of tumor cells expressing mesenchymal marker vimentin (vimentin+ CK+) was significantly higher in the SS group than that in the LS group (p=0.021). The density of E-cadherin- vimentin+ tumor cells (E-cadherin- vimentin+ CK+) was also significantly higher in the SS group (p=0.020). The density of OX40 expressing cells was significantly higher in the SS group compared to that in the LS group (p=0.006). The density of vimentin-expressing tumor cells was positively correlated with FoxP3+ CD4+ regulatory T-cells (r=0.29, p=0.047) and OX40+ cells (r=0.48, p<0.001).
Conclusion: EMT-related features were enriched in BTC patients with poor survival outcomes and associated with regulatory T-cell infiltration.
- Author(s)
- Chung Ryul Oh; Hyung-Don Kim; Yeon-Mi Ryu; Seonmin Lee; Danbee Kim; DA Sol Lee; Jae Ho Jeong; Heung-Moon Chang; Baek-Yeol Ryoo; Kyu-Pyo Kim; Miyeon Kim; Sang-Yeob Kim; Changhoon Yoo
- Issued Date
- 2023
- Type
- Article
- Keyword
- Epithelial-mesenchymal transition; biliary tract cancer; regulatory T-cell; tumor microenvironment
- DOI
- 10.21873/anticanres.16201
- URI
- https://oak.ulsan.ac.kr/handle/2021.oak/17820
- Publisher
- ANTICANCER RESEARCH
- Language
- 영어
- ISSN
- 0250-7005
- Citation Volume
- 43
- Citation Number
- 2
- Citation Start Page
- 645
- Citation End Page
- 652
-
Appears in Collections:
- Medicine > Nursing
- 공개 및 라이선스
-
- 파일 목록
-
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.