절간 고구마를 이용한 RNA 생산에 관한 연구

Abstract

RNA생산을 위한 최적 당화조건을 이용하여 생산된절간 고구당화액을 탄소원으로 효모 RNA생산능력이 가장 양호한 Cryptococcus laurentii 균주를 분리하였다.

최적 배양조건을 검토한 결과 질소원으로 ammonium sulfate 0.6%, MnSO₄0.1% 그리고 ca-pantoth-enate 400㎍/l를 무기염류, 비타민류로 각각 첨가하였을 때 최대 RNA 생산능력을 나타내었다. 기본배지의 배양조건을 검토하여 얻은 최종배지에서의 효모 균체당 RNA 생산은 30.2% 증가하였다.

During an extensive screening tests of yeast on their RNA formation it was found that Cryptococcus laurentii had especially high RNA content and high dry cell weight (D. C. W) in the case of using hydrolyzate of sliced and dried sweet potatoes as a carbon source.

Under the above conditions, the RNA content and yield of dry cells were investigated changing the media composition. Ammonium sulfate 0.6% was appeared to be favorable as a nitrogen sources. The optional concentration of Mn?² was showed to be 0.1%.

Ca-pantothenate, 400㎍/m1, showed relatively favorable effects as a growth factor. The maximum RNA content of dry cell weight was 16.8% when grown on the medium containing above supplements under the optimal conditions.

During an extensive screening tests of yeast on their RNA formation it was found that Cryptococcus laurentii had especially high RNA content and high dry cell weight (D. C. W) in the case of using hydrolyzate of sliced and dried sweet potatoes as a carbon source.

Under the above conditions, the RNA content and yield of dry cells were investigated changing the media composition. Ammonium sulfate 0.6% was appeared to be favorable as a nitrogen sources. The optional concentration of Mn?² was showed to be 0.1%.

Ca-pantothenate, 400㎍/m1, showed relatively favorable effects as a growth factor. The maximum RNA content of dry cell weight was 16.8% when grown on the medium containing above supplements under the optimal conditions.