쥐전층각막이식 모델을 이용한 각막이식거부반응 연구

Abstract

Penetrating keratoplasty in inbred Lewis rat with either Lewis donor corneas(syngeneic transplantation group) or Brown Norway donor corneas(disparate transplantation group) were performed. The kinetic changes of the inflammatory cell subtypes in the corneal transplantation site and the clinical features were studied.

All grafts(10 grafts) were remained clear in the syngeneic group. There was a significant inflammatory reaction in the wound especially surrounding the sutures. These cells were mostly macrophages(OX-42). This response in the wound progressively diminished by third week. Rare T helper/inducer cells(W3/25) were noted in the graft early. By the second week this response in the graft had resolved.

Nine grafts out of 10 were rejected by the third week in the disparate group. Macrophages were seen in moderate numbers in the graft and wound during the first week. By the second week T helper/inducer cells and T suppressor/cytotoxic cells(OX-8) Increased. In third or fourth week T suppressor/cytotoxic cells were most prominent cells in the rejected graft. The ratio of these three cell types(T suppressor/cytotoxic : T helper/inducer cell:macrophage) in the rejected cornea was 1.5:1:0.8.

Penetrating keratoplasty in inbred Lewis rat with either Lewis donor corneas(syngeneic transplantation group) or Brown Norway donor corneas(disparate transplantation group) were performed. The kinetic changes of the inflammatory cell subtypes in the corneal transplantation site and the clinical features were studied.

All grafts(10 grafts) were remained clear in the syngeneic group. There was a significant inflammatory reaction in the wound especially surrounding the sutures. These cells were mostly macrophages(OX-42). This response in the wound progressively diminished by third week. Rare T helper/inducer cells(W3/25) were noted in the graft early. By the second week this response in the graft had resolved.

Nine grafts out of 10 were rejected by the third week in the disparate group. Macrophages were seen in moderate numbers in the graft and wound during the first week. By the second week T helper/inducer cells and T suppressor/cytotoxic cells(OX-8) Increased. In third or fourth week T suppressor/cytotoxic cells were most prominent cells in the rejected graft. The ratio of these three cell types(T suppressor/cytotoxic : T helper/inducer cell:macrophage) in the rejected cornea was 1.5:1:0.8.