토끼각막내피세포의 배양에 대한 성장인자의 효과

Abstract

In order to investigate the effects of epidermal growth factor(EGF), fibroblast growth factor(FGF) and transforming growth factor-β(TGF-β), rabbit corneal endothelial cells were cultured. After adding growth factors individually or in combination, DNA synthesis of corneal endothelial cells was measured. EGF and FGF increased DNA synthesis of corneal endothelial cells at all concentration tested(0.03ng/ml-300ng/ml) compared with the control. The maximum increase of DNA synthesis was shown at the concentration of 0.3-10ng/ml of EGF or 10-30ng/ml of FGF. TGF-β reduced DNA synthesis of corneal endothelial cells at all concentration tested (0.03ng/ml-10ng/ml). DNA synthesis of corneal endothelial cells was more increased when EGF and FGF were added in combination. TGF-β inhibited DNA synthesis induced by EGF or FGF when added at the same time. These results indicate that combination of EGF and FGF has maximum effect in DNA synthesis increase of rabbit corneal endothelial cells.

In order to investigate the effects of epidermal growth factor(EGF), fibroblast growth factor(FGF) and transforming growth factor-β(TGF-β), rabbit corneal endothelial cells were cultured. After adding growth factors individually or in combination, DNA synthesis of corneal endothelial cells was measured. EGF and FGF increased DNA synthesis of corneal endothelial cells at all concentration tested(0.03ng/ml-300ng/ml) compared with the control. The maximum increase of DNA synthesis was shown at the concentration of 0.3-10ng/ml of EGF or 10-30ng/ml of FGF. TGF-β reduced DNA synthesis of corneal endothelial cells at all concentration tested (0.03ng/ml-10ng/ml). DNA synthesis of corneal endothelial cells was more increased when EGF and FGF were added in combination. TGF-β inhibited DNA synthesis induced by EGF or FGF when added at the same time. These results indicate that combination of EGF and FGF has maximum effect in DNA synthesis increase of rabbit corneal endothelial cells.