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장내 뮤신 분해균인 Akkermansia muciniphila가 상피 세포의 발달에 미치는 영향과 기전

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Abstract
Mucin-degrading bacteria are densely populated in the intestinal epithelium
however, their interaction with intestinal stem cells (ISCs) and their progeny have not been elucidated. To determine whether mucin-degrading bacteria play a role in gut homeostasis, mice were treated with Akkermansia muciniphila, a specialized species that degrades mucin. Administration of A. muciniphila for 4 weeks accelerated the proliferation of Lgr5+ ISCs and promoted the differentiation of Paneth cells and goblet cells in the small intestine (SI). I found similar effects of A. muciniphila in the colon. The levels of acetic and propionic acids were higher in the cecal contents of A. muciniphila-treated mice than in PBS-treated mice. SI organoids treated with cecal contents obtained from A. muciniphila-treated mice were larger and could be diminished by treatment with G protein-coupled receptor (Gpr) 41/43 antagonists. Pre-treatment of mice with A. muciniphila reduced gut damage caused by radiation and methotrexate. Further, a novel isotype of the A. muciniphila strain was isolated from heathy human feces that showed enhanced function in intestinal epithelial regeneration. These findings suggest that mucin-degrading bacteria (e.g., A. muciniphila) may play a crucial role in promoting ISC-mediated epithelial development and contribute to intestinal homeostasis maintenance.
Author(s)
김승일
Issued Date
2021
Awarded Date
2021-08
Type
Dissertation
Keyword
Gut microbiotaAkkermansia muciniphilaIntestinal stem cellsEpithelial development
URI
https://oak.ulsan.ac.kr/handle/2021.oak/5769
http://ulsan.dcollection.net/common/orgView/200000505590
Alternative Author(s)
SEUNGIL KIM
Affiliation
울산대학교
Department
일반대학원 의과학과 의과학전공
Advisor
권미나
Degree
Doctor
Publisher
울산대학교 일반대학원 의과학과 의과학전공
Language
eng
Rights
울산대학교 논문은 저작권에 의해 보호받습니다.
Appears in Collections:
Medical Science > 2. Theses (Ph.D)
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