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Functional regulation of macrophages phenotype by SLIT3

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Alternative Title
SLIT3 에 의한 대식세포 표현형의 기능적 조절
Abstract
Slit homolog 3 protein (SLIT3)/roundabout (ROBO) receptor axis plays a critical role in nerve cell induction, vascular necrosis, embryonic development, bone remodeling, and tumor microenvironment. However, the functional role of this axis in regulation of macrophage phenotypes has not been elucidated yet. In the current study, lipopolysaccharides (LPS)-stimulated enhanced SLIT3 expression in macrophages. We found that LPS stimulated SLIT3 expression in bone marrow macrophages (BMMs) and in turn elevated SLIT3 led to enhancement of phagocytic capacity of these cells in vitro. On the other hand, SLIT3 deficiency skewed towards macrophage 1 (M1) profile accompanied by increase in the expression of pro-inflammatory cytokines. This phenotype is linked to increased NLR family pyrin domain containing 3 (NLRP3) and adaptor molecule apoptosis-associated speck-like protein containing a CARD (ASC) in macrophages primed with LPS. Moreover, SLIT3-deficient mice displayed increased M1 population in peritoneal cells compared with that of wild-type mic followed by LPS administration in vivo. These findings suggest that the induction of SLIT3 may play an important role in the macrophage-associated inflammation, and thereby provide the potential treatment strategy for the excessive inflammation or sustained hyperinflammatory response.
Author(s)
오소윤
Issued Date
2021
Awarded Date
2021-08
Type
Dissertation
Keyword
SLIT3LPSinflammationphagocytosisMacrophag
URI
https://oak.ulsan.ac.kr/handle/2021.oak/5787
http://ulsan.dcollection.net/common/orgView/200000508005
Alternative Author(s)
Soyoon Oh
Affiliation
울산대학교
Department
일반대학원 의과학전공
Advisor
장은주
Degree
Master
Publisher
울산대학교 일반대학원 의과학전공
Language
eng
Rights
울산대학교 논문은 저작권에 의해 보호받습니다.
Appears in Collections:
Medical Science > 1. Theses (Master)
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