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Phenotypic difference of tissue-resident memory T cells in solid tumor

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Abstract
The presence and clinical importance of tissue-resident memory T (TRM) cells have been recently described in association with various cancer types. However, the frequency and phenotypic characteristics of TRM cells are largely unknown. We analyzed single-cell populations of colorectal cancer (CC, n=18), stomach cancer (SC, n=13), renal cell carcinoma (RCC, n=19), and breast cancer (BC, n=16) by dissociation of tumor tissue with collagenase/hyaluronidase. We investigated populations of naïve, effector, and memory T and TRM cells by flow cytometry. Compared with the other tumor types, CC was associated with a significantly lower percentage of CD8+ T cells (P < 0.001) among CD3+ T cells. Memory T cells were generally the dominant population. Among CD4+ cells, CC was associated with a significantly higher proportion of CD103+ T cells than other tumor types (P < 0.001). Among CD8+ cells, CC and SC were associated with higher CD103+ T cell proportions than RCC and BC (P < 0.001). Significantly more CD8+ than CD4+ cells expressed CD103 (P < 0.001). In association with SC, RCC, and BC, CD8+ T cells had a similar T cell phenotype composition pattern: fewer effector T cells and more memory-type T cells among CD103+ cells compared with CD103- cells (P < 0.05). Immune cell population composition was not significantly associated with pT stage or tumor-infiltrating lymphocyte levels. TRM cell abundance and phenotypes varied among CC, SC, RCC, and BC. Further studies regarding the clinical significance and functional differences of TRM associated with various tumors are warranted.
Author(s)
박혜선
Issued Date
2020
Awarded Date
2021-02
Type
Dissertation
Keyword
human cancersingle cellCD103tissue-resident memory T cellTRM
URI
https://oak.ulsan.ac.kr/handle/2021.oak/5788
http://ulsan.dcollection.net/common/orgView/200000363729
Alternative Author(s)
Park Hye Seon
Affiliation
울산대학교
Department
일반대학원 의과학전공
Advisor
공경엽
Degree
Master
Publisher
울산대학교 일반대학원 의과학전공
Language
eng
Rights
울산대학교 논문은 저작권에 의해 보호받습니다.
Appears in Collections:
Medical Science > 1. Theses (Master)
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