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Role of zinc in the induction of neurotoxic A1 astrocyte in primary mouse astrocyte culture

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Alternative Title
A1 성상세포 유도에서의 아연의 역할 규명
Abstract
Neurotoxic A1 astrocyte is induced by microglia-mediated neuroinflammation and plays a critical role in the neuronal damages in various neurological diseases. Therefore, factors facilitating or blocking the conversion to A1 astrocyte can be promising therapeutic targets. In this study, we demonstrate that treatment with 30μM zinc chloride converted normal astrocytes to A1 astrocytes in vitro in the absence of microglia, likely by promoting inflammatory signals in the astrocytes. FluoZin-3 live-cell imaging confirmed that neuroinflammation also induces the release of intracellular zinc from metallothionein-3 (MT3) and contributes to a positive feedback loop. Neuroinflammation-mediated A1 astrocytosis was partially attenuated when intracellular zinc is depleted by co-treatment with 1μM tetrakis (2-pyridylmethyl) ethylenediamine (TPEN), a potent zinc chelator, or genetic knockout of MT3. Collectively, our findings show that zinc plays a crucial role in the induction of A1 astrocytes by interacting with the classic neuroinflammatory pathway in a bidirectional manner.
Author(s)
김건우
Issued Date
2021
Awarded Date
2021-02
Type
Dissertation
Keyword
아연
URI
https://oak.ulsan.ac.kr/handle/2021.oak/5842
http://ulsan.dcollection.net/common/orgView/200000370137
Alternative Author(s)
Kim Keon-Woo
Affiliation
울산대학교
Department
일반대학원 의학과
Advisor
고재영
Degree
Master
Publisher
울산대학교 일반대학원 의학과
Language
eng
Rights
울산대학교 논문은 저작권에 의해 보호받습니다.
Appears in Collections:
Medicine > 1. Theses (Master)
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