Study of Enhanced Synergistic Effect of Homobifunctional Imidoester Modified ZnO for Antibiotics

Abstract

Resistance to antibiotics due to abuse and abuse is one of the world's biggest public health challenges. Although advanced nanotechnology is used in the production of antibiotics, due to side effects such as blood clotting, toxicity, low efficacy and low biocompatibility, new types of nanomaterial composite materials are needed to cope with these effects. We first introduce a simple method that can synthesize Homobifunctional Imidoester-coated nano-spindle (HINS) zinc oxide composites with the same biological functions to enhance the efficacy of antibiotics and reduce toxicity and coagulation. The antibiotic efficacy of the composite material is twice that of commercial zinc nanoparticles. In addition, due to the covalent acylation group of HI, they have good biocompatibility, increased surface charge and solubility, and produce a large amount of Zn2+ ions and defensive reactive oxygen species (ROS), which can effectively kill bacteria and fungi. In vitro and in vivo experiments, in the low-dose and low-dose treatment of intravenous administration, the synergistic effect of the combination therapy of HINS complex and itraconazole showed that the destruction rate of fungi exceeded 90%. Therefore, in the medical field, HINS composite materials can be used to reduce the abuse and the impact of antibiotic abuse. Next, BP(black phosphorus) is used as a new type of two-dimensional nanomaterials with good photothermal and drug loading properties in medical anti-tumor and biosensor research. For the first time, we tried to use the photothermal properties of black phosphorous to conduct research on disease diagnosis and other aspects, with the goal of providing more convenient and fast diagnostic techniques. Here, we use the BP and laser to irradiate the temperature to make the E. coli cleavage and split the DNA, and then use HI to bind the BP and DNA, which is more conducive to the enrichment of DNA. Compared with the commercialized QIAGEN kit and Observed by Real-time PCR technology, the extraction efficiency of DNA will be more significantly improved. This provides new ideas for the future direction of black phosphorus in the molecular diagnosis of pathogens and lays a good foundation.