KLI

Correlation of phospholipidosis between mouse liver three dimensional (3D) organoids and in vivo mouse

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Abstract
The purpose of this study was to test whether the phenomena of phospholipidosis in mouse liver organoids was identical with that in in vivo mouse toxicity studies. For this purpose, the organoids were differentiated from liver stem cells of a C57BL/6 mouse and three compounds, amiodarone, tamoxifen and thioridazine, were treated to induce DIPL to the organoids for 72 hours. After differentiation, the organoids displayed compact spherical appearance composed of uniformly polygonal cells. Furthermore, the organoids showed a notable lower expression of adult stem cell genes, whereas hepatic progenitor/biliary gene and early hepatocyte gene levels were higher than those of the liver stem cells. The electron microscopy examination showed the intracytoplasmic aggregations of the multilamellar bodies, a hallmark of DIPL, in amiodarone-treated organoids. And histological examination and gene expression associated with DIPL supported the result from electron microscopy. Our findings suggest that mouse liver organoids can reflect well the expected or well-known outcomes in phospholipidosis-induced in vivo mouse and can support further researches for predicting the DIPL in liver organoids.
Author(s)
이상준
Issued Date
2018
Awarded Date
2019-02
Type
Dissertation
URI
https://oak.ulsan.ac.kr/handle/2021.oak/6160
http://ulsan.dcollection.net/common/orgView/200000175083
Affiliation
울산대학교
Department
일반대학원 의학과의과학전공
Advisor
손우찬
Degree
Master
Publisher
울산대학교 일반대학원 의학과의과학전공
Language
eng
Rights
울산대학교 논문은 저작권에 의해 보호받습니다.
Appears in Collections:
Medical Science > 1. Theses (Master)
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