대뇌 허혈 / 재관류 모델에서 황화수소 투여 시점에 따른 신경보호 효과

Abstract

Hydrogen sulfide (NaHS) may reduce the extent of cellular damage related with cerebral ischemia/reperfusion (I/R) injury. We assumed that the NaHS administration time influences its neuroprotective effects, and aimed to prove this hypothesis using magnetic resonance imaging (MRI) and 1H magnetic resonance spectroscopy (1H-MRS). Rats were introduced to transient (60 min) middle cerebral artery occlusion (MCAO). MRI was acquired from 4 groups: sham, control (I/R), and sodium hydrosulfide (NaHS)-30 and NaHS-1 (NaHS injection 30 and 1 min before reperfusion, respectively) at baseline, 3, 9, and 24 h after ischemia. And Terminal transferase d-UTP nick-end labelling (TUNEL) assay was performed for histologic analysis including apoptosis rate at 24 h post-ischemia. In the NaHS-1 group was highest relative apparent diffusion coefficient (ADC) value and lowest relative T2 value of the peri-infarct region at 24 h post-ischemia. On serial 1H-MRS of the ischemic core and peri-infarct regions, no significant difference in the N-acetyl-L-aspartate (NAA) levels or in the combination score of NAA, glutamate, and taurine was observed between the NaHS-1 and NaHS-30 groups. Both the total infarct volume and midline shift (MLS) at 24 h post-ischemia were lowest in the NaHS-1 group. The apoptosis rate was lowest in the ischemic core and peri-infarct regions in the NaHS-1 group. The results suggest that NaHS administration timing influences its neuroprotective effects. Furthermore, this neuroprotective effect may be related to the alleviation of oedema and apoptosis and protection afforded by neuronal metabolites.