선택적 코발트 흡착능이 있는 펩타이드 기반의 폐수 중 코발트 회수 시스템

Abstract

The cell surface display system was applied for the microbial adsorption process. The adsorption and recovery of cobalt was carried out using a recombinant E.coli in which the cell surface peptide YiaT CP3 using YiaT as an anchoring motif. Absorbed cobalt was evaluated with ICP-OES varying cell and cobalt concentration. Further, for industrial application adsorption capacity of the disrupted cell by sonication and freeze-dried cell was evaluated and compared with free cell. The surface displayed E.coli with CP3 peptide showed 78.6% higher adsorption than wild E.coli. The adsorption result with free cell was highest at 1 mM concentration of cobalt and its recovery was 77.46%. As the concentration of cobalt increases, the recovery tends to decrease. The result from the disrupted cell with sonication was not remarkable. Thus disruption of the cell does not affect adsorption. Freeze-dried cells had 78.46, and 67.41% of recovery at 1 mM and 3 mM concentration of cobalt, respectively. In 5 mM and 10 mM concentration of cobalt, freeze-dried E.coli showed lower recovery compared with free cell one. With the advantage of freeze-drying cells for storage, transportation, and usage, the recovery of cobalt from wastewater in low cobalt concentration with freeze-dried cell can be valuable strategy.