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Immune Profiling of Advanced Thyroid Cancers Using Fluorescent Multiplex Immunohistochemistry

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Abstract
Background: Advanced thyroid cancers, including differentiated thyroid carcinoma (DTC) with distant metastasis, and anaplastic thyroid carcinoma (ATC), are associated with poor clinical outcomes and limited treatment options. This study aimed to determine the immune profiles of advanced thyroid cancers using fluorescent multiplex immunohistochemistry (F-MIHC) and multispectral imaging (MSI). Methods: Twenty-eight tissue samples were collected from 12 patients who had DTC with distant metastasis and from 16 with ATC. The samples were assessed using F-MIHC and MSI with antibodies against the cell surface molecules, cluster of differentiation (CD)4, CD8, programmed cell death-1 (PD-1), PD ligand 1 (PD-L1), forkhead box protein 3, and cytokeratin (CK). The expression of PD-L1 was evaluated using tumor proportion score (TPS) and combined positive score (CPS). Results: Significantly, more PD-L1-positive tumor cells (CK+PD-L1+) per mm2 were found in ATC samples than in DTC samples (183.5 vs. 0.03, p < 0.001). Lymphocyte infiltration was significantly increased in ATC compared with DTC, with significantly more PD-L1- or PD-1-positive lymphocytes in ATC samples than in DTC samples. The TPS and CPS for PD-L1 expression were negative in all DTC samples but positive in 81% and 94% of ATC samples, respectively. Conclusions: Immune profiling revealed significant differences between advanced DTC and ATC, particularly in terms of PD-L1 expression and lymphocyte infiltration. Therefore, immune profiling using F-MIHC and MSI can provide invaluable information regarding tumor microenvironments, which could help select candidates for immunotherapy.
Author(s)
안종화김미화송의연유연미송동은김상엽김태용김원배송영기전민지김원구
Issued Date
2021
Type
Article
Keyword
immune systemimmunotherapythyroid carcinomaanaplasticthyroid neoplasmimmunohistochemistry
DOI
10.1089/thy.2020.0312
URI
https://oak.ulsan.ac.kr/handle/2021.oak/6976
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_proquest_miscellaneous_2419713029&amp;context=PC&amp;vid=ULSAN&amp;lang=ko_KR&amp;search_scope=default_scope&amp;adaptor=primo_central_multiple_fe&amp;tab=default_tab&amp;query=any,contains,Immune%20Profiling%20of%20Advanced%20Thyroid%20Cancers%20Using%20Fluorescent%20Multiplex%20Immunohistochemistry&amp;offset=0&amp;pcAvailability=true
Publisher
THYROID
Location
미국
Language
영어
ISSN
1050-7256
Citation Volume
31
Citation Number
1
Citation Start Page
61
Citation End Page
67
Appears in Collections:
Medicine > Medicine
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