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Stearic acid attenuates profibrotic signalling in idiopathic pulmonary fibrosis

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Abstract
Background and objective Lipid metabolism dysregulation has been implicated in the pathogenesis of IPF; however, the roles of most lipid metabolites in lung fibrosis remain unexplored. Therefore, we aimed to identify changes in lipid metabolites in the lung tissues of IPF patients and determine their roles in pulmonary fibrosis. Methods Free fatty acids in the lung tissues of IPF patients and controls were quantified using a metabolomic approach. The roles of free fatty acids in fibroblasts or epithelial cells treated with TGF-beta 1 were evaluated using fibrotic markers. The antifibrotic role of stearic acid was also assessed in a bleomycin-induced lung fibrosis mouse model. Protein levels in cell lysates or tissues were measured by western blotting. Results The levels of stearic acid were lower in IPF lung tissues than in control lung tissues. Stearic acid significantly reduced TGF-beta 1-induced alpha-SMA and collagen type 1 expression in MRC-5 cells. Furthermore, stearic acid decreased the levels of p-Smad2/3 and ROS in MRC-5 cells treated with TGF-beta 1 and disrupted TGF-beta 1-induced EMT in Beas-2B cells. Stearic acid reduced the levels of bleomycin-induced hydroxyproline in a mouse model. Conclusion Changes in the free fatty acid profile, including low levels of stearic acid, were observed in IPF patients. Stearic acid may exert antifibrotic activity by regulating profibrotic signalling.
Author(s)
송진우HA EUN SONGHAK-SU KIMHYUN JU YOOJAE OK LEEJUNG JIN HWANGKWANG MIN LEESU JUNG KIM
Issued Date
2021
Type
Article
Keyword
AnalysisB cellsfatty acididiopathic pulmonary fibrosisMetabolitesmetabolomicsnon‐esterifiedPulmonary fibrosisSaturated fatty acidstherapyTransforming growth factors
DOI
10.1111/resp.13949
URI
https://oak.ulsan.ac.kr/handle/2021.oak/6999
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_proquest_miscellaneous_2449180976&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Stearic%20acid%20attenuates%20profibrotic%20signalling%20in%20idiopathic%20pulmonary%20fibrosis&offset=0&pcAvailability=true
Publisher
RESPIROLOGY
Location
오스트레일리아
Language
영어
ISSN
1323-7799
Citation Volume
26
Citation Number
3
Citation Start Page
255
Citation End Page
263
Appears in Collections:
Medicine > Medicine
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