KLI

The arachidonic acid metabolite 11,12-epoxyeicosatrienoic acid alleviates pulmonary fibrosis

Metadata Downloads
Abstract
Epoxyeicosatrienoic acids (EETs) are metabolites of arachidonic acid that are rapidly metabolized into diols by soluble epoxide hydrolase (sEH). sEH inhibition has been shown to increase the biological activity of EETs, which are known to have anti-inflammatory properties. However, the role of EETs in pulmonary fibrosis remains unexplored. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to analyze EETs in the lung tissues of patients with idiopathic pulmonary fibrosis (IPF, n = 29) and controls (n = 15), and the function of 11,12-EET was evaluated in in vitro and in vivo in pulmonary fibrosis models. EET levels in IPF lung tissues, including those of 8,9-EET, 11,12-EET, and 14,15-EET, were significantly lower than those in control tissues. The 11,12-EET/11,12-DHET ratio in human lung tissues also differentiated IPF from control tissues. 11,12-EET significantly decreased transforming growth factor (TGF)-beta 1-induced expression of alpha-smooth muscle actin (SMA) and collagen type-I in MRC-5 cells and primary fibroblasts from IPF patients. sEH-specific siRNA and 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea (TPPU; sEH inhibitor) also decreased TGF-beta 1-induced expression of alpha-SMA and collagen type-I in fibroblasts. Moreover, 11,12-EET and TPPU decreased TGF-beta 1-induced p-Smad2/3 and extracellular-signal-regulated kinase (ERK) expression in primary fibroblasts from patients with IPF and fibronectin expression in Beas-2B cells. TPPU decreased the levels of hydroxyproline in the lungs of bleomycin-induced mice. 11,12-EET or sEH inhibitors could inhibit pulmonary fibrosis by regulating TGF-beta 1-induced profibrotic signaling, suggesting that 11,12-EET and the regulation of EETs could serve as potential therapeutic targets for IPF treatment.

Pulmonary fibrosis: Suppressing creation of scar tissue Signaling molecules called eicosanoids, which are derived from fatty acids, can suppress lung damage in idiopathic pulmonary fibrosis (IPF), a chronic, progressive disease in which scar tissue builds up in the lungs, making it hard to breathe. The causes of IPF are unknown. Eicosanoids, which have anti-inflammatory properties, have been studied in various lung diseases. Jin Woo Song at the University of Ulsan College of Medicine in Seoul, South Korea, and co-workers investigated how they might affect IPF. They found that eicosanoid levels were lower in lung tissues from patients with IPF than in healthy tissues. Further investigation showed eicosanoid levels could be boosted by suppressing an enzyme called sEH that degrades them. Thus, suppression of sEH and boosting of eicosanoid levels show promise as therapeutic targets for IPF.
Author(s)
송진우유현주이상은Gi Won HwangHak Su KimSu-Jin Moon
Issued Date
2021
Type
Article
Keyword
ActinAnti-inflammatory agentsArachidonic acidBiological activityBleomycinChronic inflammationCollagenEicosanoidsEpoxide hydrolaseExtracellular signal-regulated kinaseFatty acidsFibroblastsFibronectinFibrosisHydroxyprolineInflammationLiquid chromatographyLung diseasesLungsMass spectroscopyPulmonary fibrosisRespiratory tract diseasessiRNASmad2 proteinSmooth muscleStaphylococcal enterotoxin HTherapeutic applicationsTherapeutic targetsTransforming growth factor-b1Urea생화학
DOI
10.1038/s12276-021-00618-7
URI
https://oak.ulsan.ac.kr/handle/2021.oak/7052
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_9785405&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,The%20arachidonic%20acid%20metabolite%2011,12-epoxyeicosatrienoic%20acid%20alleviates%20pulmonary%20fibrosis&offset=0&pcAvailability=true
Publisher
EXPERIMENTAL AND MOLECULAR MEDICINE
Location
미국
Language
영어
ISSN
1226-3613
Citation Volume
53
Citation Number
5
Citation Start Page
864
Citation End Page
874
Appears in Collections:
Medicine > Medicine
Authorize & License
  • Authorize공개
Files in This Item:
  • There are no files associated with this item.

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.