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Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial development

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Abstract
Mucin-degrading bacteria are densely populated in the intestinal epithelium; however, their interaction with intestinal stem cells (ISCs) and their progeny have not been elucidated. To determine whether mucin-degrading bacteria play a role in gut homeostasis, mice were treated with Akkermansia muciniphila, a specialized species that degrades mucin. Administration of A. muciniphila for 4 weeks accelerated the proliferation of Lgr5(+) ISCs and promoted the differentiation of Paneth cells and goblet cells in the small intestine (SI). We found similar effects of A. muciniphila in the colon. The levels of acetic and propionic acids were higher in the cecal contents of A. muciniphila-treated mice than in PBS-treated mice. SI organoids treated with cecal contents obtained from A. muciniphila-treated mice were larger and could be diminished by treatment with G protein-coupled receptor (Gpr) 41/43 antagonists. Pre-treatment of mice with A. muciniphila reduced gut damage caused by radiation and methotrexate. Further, a novel isotype of the A. muciniphila strain was isolated from heathy human feces that showed enhanced function in intestinal epithelial regeneration. These findings suggest that mucin-degrading bacteria (e.g., A. muciniphila) may play a crucial role in promoting ISC-mediated epithelial development and contribute to intestinal homeostasis maintenance.
Author(s)
김승일신윤찬김태영김예지이용수이수현김미나오은주김광순권미나
Issued Date
2021
Type
Article
Keyword
Gut microbiotaakkermansia muciniphilaepithelial developmentg protein-coupled receptorsintestinal stem cellslgr5mucin-degrading bacteriaorganoidssmall intestine.
DOI
10.1080/19490976.2021.1892441
URI
https://oak.ulsan.ac.kr/handle/2021.oak/7304
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7946046&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Mucin%20degrader%20Akkermansia%20muciniphila%20accelerates%20intestinal%20stem%20cell-mediated%20epithelial%20development&offset=0&pcAvailability=true
Publisher
GUT MICROBES
Location
미국
Language
영어
ISSN
1949-0976
Citation Volume
13
Citation Number
1
Citation Start Page
0
Citation End Page
0
Appears in Collections:
Medicine > Medicine
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