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Influence of pretreatment of insulin on the phosphorylation of extracellular receptor kinase by gonadotropin-releasing hormone and gonadotropins in cultured human granulosa cells

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Abstract
Objective: To investigate the influence of pretreatment of insulin on the phosphorylation of ERK1/2 by gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) in cultured human granulosa cells. Study design: Human granulosa cells were collected from 20 women (age: 20-35 years) who underwent controlled ovarian hyperstimulation for in vitro fertilization and embryo transfer at Asan Medical Center (Seoul, South Korea). The presence of the receptors for insulin, GnRH, FSH, and LH in human granulosa cells was identified by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The granulosa cells were treated with 10 nM insulin or 10 nM GnRH for 5 min or 30 min and with 10 nM FSH or 10 nM LH for 24 h or 48 h. The cells were also pretreated with insulin for 30 min prior to treatment with GnRH, FSH, or LH. Western blot analysis was used to analyze ERK1/2 phosphorylation. Results: RT-PCR showed that the receptors for insulin, GnRH, FSH, and LH were expressed in human granulosa cells. Insulin, GnRH, FSH, and LH could activate ERK1/2 phosphorylation. Pretreatment with insulin inhibited ERK1/2 phosphorylation induced by GnRH and FSH while augmenting ERK1/2 phosphorylation induced by LH. Conclusions: Insulin might have a negative effect on GnRH and FSH regulation by attenuating the action of GnRH and FSH in the phosphorylation of ERK1/2 in human granulosa cells. In contrast, insulin might have a positive effect on LH regulation by potentiating the action of LH in the phosphorylation of ERK1/2. Our results showed that insulin is clearly an important regulator of human reproductive function at the ovarian level. (c) 2021 Elsevier B.V. All rights reserved.
Author(s)
고유리김주희이사라김성훈채희동
Issued Date
2021
Type
Article
Keyword
Extracellular receptor kinaseFollicle-stimulating hormoneGonadotropin-releasing hormoneHuman granulosa cellsInsulinLuteinizing hormone
DOI
10.1016/j.ejogrb.2021.05.016
URI
https://oak.ulsan.ac.kr/handle/2021.oak/7524
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_proquest_miscellaneous_2529924639&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Influence%20of%20pretreatment%20of%20insulin%20on%20the%20phosphorylation%20of%20extracellular%20receptor%20kinase%20by%20gonadotropin-releasing%20hormone%20and%20gonadotropins%20in%20cultured%20human%20granulosa%20cells&offset=0
Publisher
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY
Location
아일랜드
Language
영어
ISSN
0301-2115
Citation Volume
262
Citation Number
7
Citation Start Page
113
Citation End Page
117
Appears in Collections:
Medicine > Medicine
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