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Anti-inflammatory and anti-apoptotic effects of N-acetylcysteine in diabetic rat corneal epithelium

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Abstract
● AIM: To characterize the anti-inflammatory and antiapoptotic
effects of N-acetylcysteine (NAC) in streptozotocin
(STZ)-induced diabetic rat corneal epithelium and human
corneal epithelial cells (HCECs) exposed to a high-glucose
environment.
● METHODS: HCECs were incubated in 0, 5, 50 mmol/L
glucose medium, or 50 mmol/L glucose medium with NAC
for 24h. Diabetes was induced in rats by intraperitoneal
injection of 65 mg/kg STZ and some of these rats were
topically administered NAC to corneas with 3 mice per
group. We characterized receptor for advanced glycation
end-products (RAGE) expression using immunofluorescence,
and interleukin (IL)-1β and cleaved caspase-3 (CCAP-3)
expression using immunohistochemistry. Circulating tumor
necrosis factor (TNF)-α concentration was measured by
ELISA and cleaved poly-ADP ribose polymerase (PARP)
concentration was quantified by Western blotting. Apoptotic
cells were detected using TUNEL assay and annexin V and
propidium iodide staining.
● RESULTS: Diabetic rats had higher expression of RAGE
(2.46±0.13 fold), IL-1β, and CCAP-3 in apoptotic cells of
their corneas than control rats. The expression of RAGE
(1.83±0.11 fold), IL-1β, and CCAP-3, and the number of
apoptotic cells, were reduced by topical NAC treatment.
HCECs incubated in 50 mmol/L glucose medium showed
high concentrations of TNF-α (310±2.00 pg/mL) and
cleaved PARP (7.43±0.56 fold), and more extensive
apoptosis than cells in 50 mmol/L glucose medium.
However, the addition of NAC reduced the concentrations of
TNF-α (153.67±2.31 pg/mL) and cleaved PARP (5.55±0.31
fold) and the number of apoptotic cells.
● CONCLUSION: NAC inhibits inflammation and apoptosis
in the corneas of diabetic rats and HCECs maintained in a
high-glucose environment.
Author(s)
강순숙김재용박지연박진형이훈정호석차흥원황새벽
Issued Date
2021
Type
Article
Keyword
apoptosiscorneal epitheliumdiabetesinflammationn-acetylcysteinerat
DOI
10.18240/ijo.2021.12.01
URI
https://oak.ulsan.ac.kr/handle/2021.oak/7689
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_doaj_primary_oai_doaj_org_article_66875ae847054e169558252a892fc915&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Anti-inflammatory%20and%20anti-apoptotic%20effects%20of%20N-acetylcysteine%20in%20diabetic%20rat%20corneal%20epithelium&offset=0&pcAvailability=true
Publisher
International Journal of Ophthalmology
Location
중국
Language
영어
ISSN
2222-3959
Citation Volume
14
Citation Number
12
Citation Start Page
1805
Citation End Page
1812
Appears in Collections:
Medicine > Medicine
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