SARS-CoV-2-Specific Antibody and T Cell Response Kinetics According to Symptom Severity
- Abstract
- Data on the longevity of humoral and cell-mediated immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in patients with coronavirus disease 2019 (COVID-19) are limited. We evaluated the detailed kinetics of antibody and T-cell responses at the acute, convalescent, and post-convalescent phases in COVID-19 patients with a wide range of severity. We enrolled patients with COVID-19 prospectively from four hospitals and one community treatment center between February 2020 and January 2021. symptom severity was classified as mild, moderate, or severe/critical. Patient blood samples were collected at 1 week (acute), 1 month (convalescent), and 2 months after symptom onset (post-convalescent). Human SARS-CoV-2 IgG and IgM antibodies were measured using in-house-developed ELISA. The SARS-CoV-2-specific T-cell responses against overlapping peptides of spike proteins and nucleoprotein were measured by interferon-g enzyme-linked immunospot assays. Twenty-five COVID-19 patients were analyzed (mild, n = 5; moderate, n = 9; severe/critical, n = 11). IgM and IgG antibody responses peaked at 1 month after symptom onset and decreased at 2 months. IgG response levels were significantly greater in the severe/critical group compared with other groups. Interferon-g-producing T-cell responses increased between 1 week and 1 month after symptom onset, and had a trend toward decreasing at 2 months, but did not show significant differences according to severity. Our data indicate that SARS-CoV-2-specific antibody responses were greater in those with severe symptoms and waned after reaching a peak around 1 month after symptom onset. However, SARS-CoV-2-specific T-cell responses were not significantly different according to symptom severity, and decreased slowly during the post-convalescent phase.
- Author(s)
- 권지수; 김민재; 김민철; 김백남; 김성한; 김지연; 배성만; 신의철; 이명진; 임준서; 정지원; 정진원; 차혜희
- Issued Date
- 2021
- Type
- Article
- Keyword
- Acute Disease; Adult; Aged; Antibodies; Antibodies; Neutralizing - blood; Antibodies; Viral - immunology; Convalescence; Coronaviruses; COVID-19; COVID-19 - blood; COVID-19 - immunology; COVID-19 - pathology; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunoglobulin G - blood; Immunoglobulin M - blood; Interferon-gamma - analysis; Kinetics; Male; Middle Aged; Prospective Studies; SARS-CoV-2 - immunology; Severe acute respiratory syndrome coronavirus 2; Severity of Illness Index; T-Lymphocytes - immunology
- DOI
- 10.4269/ajtmh.20-1594
- URI
- https://oak.ulsan.ac.kr/handle/2021.oak/8055
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_proquest_miscellaneous_2543445989&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,SARS-CoV-2-Specific%20Antibody%20and%20T%20Cell%20Response%20Kinetics%20According%20to%20Symptom%20Severity&offset=0&pcAvailability=true
- Publisher
- AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
- Location
- 미국
- Language
- 한국어
- ISSN
- 0002-9637
- Citation Volume
- 105
- Citation Number
- 2
- Citation Start Page
- 395
- Citation End Page
- 400
-
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