KLI

Urine beta-2-glycoprotein 1 as a biomarker for diagnosis of systemic lupus erythematosus

Metadata Downloads
Abstract
Objective

The need for a biomarker with robust sensitivity and specificity in diagnosing systemic lupus erythematosus (SLE) remains unmet. Compared with blood samples, urine samples are more easily collected; thus, we aimed to identify such a biomarker based on urinary proteomics which could distinguish patients with SLE from healthy controls (HCs).

Methods

Urine samples were collected from 76 SLE patients who visited rheumatology clinic in 2019 at Asan medical center and from 25 HCs. Urine proteins were analyzed using sequential windowed acquisition of all theoretical fragment ion spectra-mass spectrometry, and the candidate marker was confirmed by enzyme-linked immunosorbent assay (ELISA). Receiver operating characteristic curve analysis was used to determine the diagnostic value of the candidate biomarker.

Results

Of 1157 proteins quantified, 153 were differentially expressed in urine samples from HCs. Among them were previously known markers including alpha-1-acid glycoprotein 1, alpha-2-HS-glycoprotein, ceruloplasmin, and prostaglandin-H2 D-isomerase. Moreover, the amount of beta-2 glycoprotein (APOH) was increased in the urine of patients with SLE. The ELISA results also showed the level of urine APOH was higher in patients with SLE than in HCs and patients with rheumatoid arthritis. Moreover, the level was not different between SLE patients with and without nephritis. The urine APOH had an area under the curve value of 0.946 at a cut-off value of 228.53 ng/mg (sensitivity 91.5%, specificity 92.0%) for the diagnosis of SLE.

Conclusion

The results indicate that the urine APOH level can be an appropriate screening tool in a clinical setting when SLE is suspected.
Author(s)
김경곤김용길오지선유빈이은주이정선이창근홍석찬jeonghyn Yeom
Issued Date
2021
Type
Article
Keyword
beta 2-Glycoprotein IBiomarkersHumansLupus ErythematosusSystemic - diagnosisOrosomucoidROC Curve
DOI
10.1177/09612033211014268
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8129
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_proquest_miscellaneous_2524865550&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Urine%20beta-2-glycoprotein%201%20as%20a%20biomarker%20for%20diagnosis%20of%20systemic%20lupus%20erythematosus&offset=0&pcAvailability=true
Publisher
LUPUS
Location
영국
Language
한국어
ISSN
0961-2033
Citation Volume
30
Citation Number
8
Citation Start Page
1306
Citation End Page
1313
Appears in Collections:
Medicine > Medicine
Authorize & License
  • Authorize공개
Files in This Item:
  • There are no files associated with this item.

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.