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Acetylated Diacylglycerol 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol in Autoimmune Arthritis and Interstitial Lung Disease in SKG Mice

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Abstract
Acetylated diacylglycerol 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG) is a lipid molecule from the antlers of sika deer that might reduce inflammation by effectively controlling neutrophil infiltration, endothelial permeability and inflammatory chemokine production. Therefore, we evaluated the modulatory effect of PLAG on arthritis and interstitial lung disease (ILD) in an autoimmune arthritis model. We injected curdlan into SKG mice and PLAG was orally administered every day from 3 weeks to 20 weeks after the curdlan injection. The arthritis score was measured every week after the curdlan injection. At 20 weeks post-injection, the lung specimens were evaluated with HE, Masson's trichrome and multiplexed immunofluorescent staining. Serum cytokines were also analyzed using a Luminex multiple cytokine assay. PLAG administration decreased the arthritis score until 8 weeks after the curdlan injection. However, the effect was not sustained thereafter. A lung histology revealed severe inflammation and fibrosis in the curdlan-induced SKG mice, which was attenuated in the PLAG-treated mice. Furthermore, immunofluorescent staining of the lung tissue showed a GM-CSF+ neutrophil accumulation and a decreased citrullinated histone 3 expression after PLAG treatment. PLAG also downregulated the levels of IL-6 and TNF-alpha and upregulated the level of sIL-7R alpha, an anti-fibrotic molecule. Our results indicate that PLAG might have a preventative effect on ILD development through the resolution of NETosis in the lung.
Author(s)
김도훈김용길유빈윤재희이은주이제현이창근이희섭임두호정미령홍석찬
Issued Date
2021
Type
Article
Keyword
AntibodiesAntlersArthritisautoimmuneAutoimmune diseasesCitrullineCytokinesDiacylglycerolFibrosisGlycerolGranulocyte-macrophage colony-stimulatingfactorHistonesInflammationInjectionInterleukin 6interstitial lung diseaseLeukocytes (neutrophilic)Life sciencesLung diseasesLymphocytesMortalityMutationNETosisNeutrophilsOral administrationPermeabilityPLAGPolymersSignal transduction Tumornecrosis factor-TNFTumor necrosis factor-α
DOI
10.3390/biomedicines9091095
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8133
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_doaj_primary_oai_doaj_org_article_6a4e39fadcd549fb9ce8a68941240781&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Acetylated%20Diacylglycerol%201-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol%20in%20Autoimmune%20Arthritis%20and%20Interstitial%20Lung%20Disease%20in%20SKG%20Mice&offset=0&pcAvailability=true
Publisher
BIOMEDICINES
Location
미국
Language
한국어
ISSN
2227-9059
Citation Volume
9
Citation Number
9
Citation Start Page
0
Citation End Page
0
Appears in Collections:
Medicine > Medicine
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