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Rapid COVID-19 Molecular Diagnostic System Using Virus Enrichment Platform

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Abstract
The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus (SARS-CoV)-2, is rapidly spreading and severely straining the capacities of public health communities and systems around the world. Therefore, accurate, rapid, and robust diagnostic tests for COVID-19 are crucial to prevent further spread of the infection, alleviate the burden on healthcare and diagnostic facilities, and ensure timely therapeutic intervention. To date, several detection methods based on nucleic acid amplification have been developed for the rapid and accurate detection of SARS-CoV-2. Despite the myriad of advancements in the detection methods for SARS-CoV-2, rapid sample preparation methods for RNA extraction from viruses have rarely been explored. Here, we report a rapid COVID-19 molecular diagnostic system that combines a self-powered sample preparation assay and loop-mediated isothermal amplification (LAMP) based naked-eye detection method for the rapid and sensitive detection of SARS-CoV-2. The self-powered sample preparation assay with a hydrophilic polyvinylidene fluoride filter and dimethyl pimelimidate can be operated by hand, without the use of any sophisticated instrumentation, similar to the reverse transcription (RT)-LAMP-based lateral flow assay for the naked-eye detection of SARS-CoV-2. The COVID-19 molecular diagnostic system enriches the virus population, extracts and amplifies the target RNA, and detects SARS-CoV-2 within 60 min. We validated the accuracy of the system by using 23 clinical nasopharyngeal specimens. We envision that this proposed system will enable simple, facile, efficient, and inexpensive diagnosis of COVID-19 at home and the clinic as a pre-screening platform to reduce the burden on the medical staff in this pandemic era.
Author(s)
구본한권지수김명규김성한김지연김현수신용이효주장윤옥차혜희
Issued Date
2021
Type
Article
Keyword
COVID-19molecular diagnosticsrapid diagnosticssample preparationvirus enrichment
DOI
10.3390/bios11100373
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8225
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_doaj_primary_oai_doaj_org_article_973b8a3fff604cf8945c046272cecfcb&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Rapid%20COVID-19%20Molecular%20Diagnostic%20System%20Using%20Virus%20Enrichment%20Platform&offset=0&pcAvailability=true
Publisher
BIOSENSORS-BASEL
Location
스위스
Language
한국어
ISSN
2079-6374
Citation Volume
11
Citation Number
10
Citation Start Page
0
Citation End Page
0
Appears in Collections:
Medicine > Medicine
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