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PD-L1 expression in paired biopsies and surgical specimens in gastric adenocarcinoma: A digital image analysis study

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Abstract
Programmed death-ligand 1 (PD-L1) expression in biopsies of gastric carcinoma may predict the results in corresponding surgical specimens. We compared PD-L1 immunohistochemistry (IHC) 22C3 pharmDx expression in paired biopsy and resection specimens. We also characterized the validity of a new PD-L1 assay using digital image analysis.

PD-L1 IHC with 22C3 pharmDx and clone 73 10 was performed in 224 gastric cancer tissues (112 biopsies and paired surgical tissues) and the specimens were analyzed with the Leica Aperio Imagescope. For statistical analyses, the area under the receiver operating characteristic curve and R package were used.

With 22C3 pharmDx, a PD-L1 combined positive score of >= 1 was found in 36 biopsied (32.14 %) and 53 surgical (47.32 %) samples. PD-L1 expression results were concordant in 71 cases (63.4 %) and discordant in 41 (36.6 %). The overall discordance rate was 36.61 % (95 % confidence interval 2.101-8.983) and the. value was 0.254 with fair agreement. The sensitivity and specificity of biopsy PD-L1 to predict the results of the surgical specimen was 62 % and 73 %, respectively. The correlation of 22C3 pharmDx and clone 73 10 was high (correlation coefficient = 0.88). When only tumor cell staining was compared, this correlation was increased (correlation coefficient = 0.95).

Our results indicated moderate association of PD-L1 expression between gastric biopsies and corresponding resected tumors. Results of PD-L1 assay with 73 10 are comparable to 22C3 pharmDx results.
Author(s)
허유정김빛나리김현진김소이장민선김경미
Issued Date
2021
Type
Article
Keyword
PD-L1Gastric carcinomaBiopsySurgeryDigital image analysis
DOI
10.1016/j.prp.2020.153338
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8297
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_proquest_miscellaneous_2478033591&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,PD-L1%20expression%20in%20paired%20biopsies%20and%20surgical%20specimens%20in%20gastric%20adenocarcinoma:%20A%20digital%20image%20analysis%20study&offset=0&pcAvailability=true
Publisher
PATHOLOGY RESEARCH AND PRACTICE
Location
독일
Language
영어
ISSN
0344-0338
Citation Volume
218
Citation Start Page
0
Citation End Page
0
Appears in Collections:
Medicine > Medicine
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