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Antinociceptive and Anti-Inflammatory Effects of Recombinant Crotamine in Mouse Models of Pain

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Abstract
Crotamine, a toxin found in the venom of the South American rattlesnake Crotalus durissus terrificus, has been reported to have antinociceptive effects. We purified recombinant crotamine expressed in Escherichia coli and investigated its antinociceptive and anti-inflammatory effects using the hot-plate test, acetic-acid-induced writhing method, and formalin test in mice. Recombinant crotamine was administered intraperitoneally (0.04-1.2 mg kg(-1)) or intraplantarly (0.9-7.5 mu g 10 mu L-1) before the tests. The paw volume was measured with a plethysmometer. To evaluate the antagonistic and anti-inflammatory effects of naloxone, subcutaneous naloxone (4 mg kg(-1)) or intraplantar naloxone (5 mu g 10 mu L-1) was administered before recombinant crotamine. For tumor necrosis factor (TNF)-alpha assays, blood was drawn 3 h after formalin injection and measured using enzyme-linked immunosorbent assay. Intraperitoneal and intraplantar recombinant crotamine had antinociceptive and anti-inflammatory effects, neither of which were affected by pre-treatment with naloxone. The mean serum TNF-alpha levels were significantly lower in the intraperitoneal recombinant crotamine (0.4 and 1.2 mg kg(-1)) or intraplantar (2.5 and 7.5 mu g 10 mu L-1) recombinant crotamine groups than in the saline group and were not affected by naloxone pre-treatment. In conclusion, recombinant crotamine possesses significant antinociceptive and anti-inflammatory effects that do not appear to be related to the opioid receptor. The antinociceptive and anti-inflammatory effects of intraperitoneal or intraplantar recombinant crotamine are related to TNF-alpha.
Author(s)
박종연Bich Hang Do이주승양현철Anh Ngoc NguyenMNartin Krupa김종재장연진최한석
Issued Date
2021
Type
Article
Keyword
crotaminenociceptioninflammationnaloxonetumor necrosis factor-α
DOI
10.3390/toxins13100707
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8305
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_doaj_primary_oai_doaj_org_article_645d44c31e3746069d387c6555f52d03&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Antinociceptive%20and%20Anti-Inflammatory%20Effects%20of%20Recombinant%20Crotamine%20in%20Mouse%20Models%20of%20Pain&offset=0&pcAvailability=true
Publisher
TOXINS
Location
대한민국
Language
영어
ISSN
2072-6651
Citation Volume
13
Citation Number
10
Citation Start Page
0
Citation End Page
0
Appears in Collections:
Medicine > Medicine
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