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Diagnostic performance of real-time polymerase chain reaction assay on blood for invasive aspergillosis and mucormycosis

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Abstract
Objectives
This study aimed to evaluate the diagnostic usefulness of real-time (RT) polymerase chain reaction (PCR) on blood samples for diagnosis of invasive aspergillosis and mucormycosis in patients with suspected invasive mould infection.

Methods
Adult patients with suspected invasive mould infection were prospectively enrolled at a tertiary referral hospital in Seoul, South Korea between 2017 and 2020. Standard tests for diagnosis of invasive mould infection and RT-PCR for Aspergillus, Mucor and Rhizopus using blood samples were performed. We evaluated the diagnostic performance of RT-PCR tests in patients diagnosed with proven and probable invasive aspergillosis or mucormycosis infection, according to the modified definitions of the EORTC/MSG 2019.

Results
A total of 102 patients with suspected invasive mould infection were enrolled. Of these patients, 46 (45%) were classified as having proven (n = 13) or probable (n = 33) invasive aspergillosis, 21 (21%) as proven (n = 17) or probable (n = 4) invasive mucormycosis and 18 (18%) as possible invasive mould infection. The remaining 13 (13%) were classified as not having invasive mould infection. Patients with possible invasive mould infection (n = 18) and coinfection of aspergillosis and mucormycosis (n = 4) were excluded from the final analysis. The sensitivity and specificity of the Aspergillus PCR were 54.3% ([25/46], 95% confidence interval [CI]: 40.2?67.9%) and 94.1% ([32/34], 95% CI: 80.9?98.4%), respectively. The sensitivity and specificity of the Mucor or Rhizopus PCR were 57.1% ([12/21], 95% CI: 36.6?75.5%) and 76.3% ([45/59], 95% CI: 64.0?85.3), respectively.

Conclusions
Our study suggests that blood PCR can be a useful adjunct test for diagnosing patients with suspected invasive mould infection.
Author(s)
김민재김성한김양수김지연서현지손효주이상오정용필정지원최상호
Issued Date
2021
Type
Article
Keyword
aspergillosisgalactomannanmouldmucormycosisreal-time polymerase chain reaction
DOI
10.1111/myc.13319
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8317
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_proquest_miscellaneous_2529943637&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Diagnostic%20performance%20of%20real-time%20polymerase%20chain%20reaction%20assay%20on%20blood%20for%20invasive%20aspergillosis%20and%20mucormycosis&offset=0&pcAvailability=true
Publisher
MYCOSES
Location
EU(유럽연합)
Language
한국어
ISSN
0933-7407
Citation Volume
64
Citation Number
12
Citation Start Page
1554
Citation End Page
1562
Appears in Collections:
Medicine > Medicine
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