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A DNA Topoisomerase II Inhibitor Results in Ex Vivo Differentiation of THP-1 Cells and Activation of Dendritic Cells

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Abstract
Abstract
Background/aim: Effective ex vivo maturation of dendritic cells (DCs) can increase the efficiency of cancer immunotherapy. We aimed to identify novel chemicals with the potential to differentiate and activate immature DCs (iDCs) to mature DCs (mDCs).

Materials and methods: The expression of surface markers on THP-1 monocytes treated with the screened compounds was analyzed using FACS. Subsequent DC subset analysis and secreted cytokine profiling were also performed.

Results: FACS analysis showed that THP-1 cells treated with amsacrine hydrochloride, a DNA topoisomerase II inhibitor, exhibited the typical phenotype of conventional DCs (cDCs). The expression of DC activation markers was also increased after amsacrine treatment. The profile of cytokines produced by THP-1 cells treated with amsacrine was similar to that of mDCs.

Conclusion: Amsacrine has an ex vivo capability of differentiating THP-1 monocytes into cDCs. As amsacrine has been used as a stable chemotherapeutic agent in humans, it can be useful for producing mDCs for cancer immunotherapy.
Author(s)
공경엽김지형박인아이희재이희진조영진
Issued Date
2021
Type
Article
Keyword
Cell DifferentiationDendritic Cells - drug effectsHumansTHP-1 Cells - drug effectsTopoisomerase II Inhibitors - pharmacologyTopoisomerase II Inhibitors - therapeutic use
DOI
10.21873/anticanres.15428
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8355
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_crossref_primary_10_21873_anticanres_15428&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,A%20DNA%20Topoisomerase%20II%20Inhibitor%20Results%20in%20Ex%20Vivo%20Differentiation%20of%20THP-1%20Cells%20and%20Activation%20of%20Dendritic%20Cells&offset=0&pcAvailability=true
Publisher
ANTICANCER RESEARCH
Location
그리스
Language
영어
ISSN
0250-7005
Citation Volume
41
Citation Number
12
Citation Start Page
6087
Citation End Page
6094
Appears in Collections:
Medicine > Medicine
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