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Identification of 8-Digit HLA-A, -B, -C, and-DRB1 Allele and Haplotype Frequencies in Koreans Using the One Lambda AllType Next-Generation Sequencing Kit

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Abstract
Background: Recent studies have successfully implemented next-generation sequencing (NGS) in HLA typing. We performed HLA NGS in a Korean population to estimate HLA-A, -B, -C, and -DRB1 allele and haplotype frequencies up to an 8-digit resolution, which might be useful for an extended application of HLA results.

Methods: A total of 128 samples collected from healthy unrelated Korean adults, previously subjected to Sanger sequencing for 6-digit HLA analysis, were used. NGS was performed for HLA-A, -B, -C, and -DRB1 using the AllType NGS kit (One Lambda, West Hills, CA, USA), Ion Torrent S5 platform (Thermo Fisher Scientific, Waltham, MA, USA), and Type Steam Visual NGS analysis software (One Lambda).

Results: Eight HLA alleles showed frequencies of >= 10% in the Korean population, namely, A*24:02:01:01 (19.5%), A*33:03:01 (15.6%), A*02:01:01:01 (14.5%), A*11:01:01:01 (13.3%), B*15:01:01:01 (10.2%), C*01:02:01 (19.9%), C*03:04:01:02 (11.3%), and DRB1*09:01:02 (10.2%). Nine previous 6-digit HLA alleles were further identified as two or more 8-digit HLA alleles. Of these, eight alleles (A*24:02:01, B*35:01:01, B*40:01:02, B*55:02:01, B*58:01:01, C*03:02:02, C*07:02:01, and DRB1*07:01:01) were identified as two 8-digit HLA alleles, and one allele (B*51:01:01) was identified as three 8-digit HLA alleles. The most frequent four-loci haplotype was H LA-A *33:03:01-B *44:03:01:01-C * 14: 03-DRB1*13:02:01.

Conclusions: We identified 8-digit HLA-A, -B, -C, and -DRB1 allele and haplotype frequencies in a healthy Korean population using NGS. These new data can be used as a representative Korean data for further disease-related HLA type analysis.
Author(s)
양정석오흥범채정돈최성은최원호황상현
Issued Date
2021
Type
Article
Keyword
8-digit HLA allelesallele frequencyDiagnostic Immunologyhaplotype frequencyHuman leukocyte antigen (HLA)Korean populationNext-generation sequencingOriginal병리학
DOI
10.3343/alm.2021.41.3.310
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8569
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_9791055&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Identification%20of%208-Digit%20HLA-A,%20-B,%20-C,%20and-DRB1%20Allele%20and%20Haplotype%20Frequencies%20in%20Koreans%20Using%20the%20One%20Lambda%20AllType%20Next-Generation%20Sequencing%20Kit&offset=0&pcAvailability=true
Publisher
ANNALS OF LABORATORY MEDICINE
Location
대한민국
Language
한국어
ISSN
2234-3806
Citation Volume
41
Citation Number
3
Citation Start Page
310
Citation End Page
317
Appears in Collections:
Medicine > Medicine
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