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Neutrophil-Selective Fluorescent Probe Development through Metabolism-Oriented Live-Cell Distinction

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Abstract
Human neutrophils are the most abundant leukocytes and have been considered as the first line of defence in the innate immune system. Selective imaging of live neutrophils will facilitate the in situ study of neutrophils in infection or inflammation events as well as clinical diagnosis. However, small-molecule-based probes for the discrimination of live neutrophils among different granulocytes in human blood have yet to be reported. Herein, we report the first fluorescent probe NeutropG for the specific distinction and imaging of active neutrophils. The selective staining mechanism of NeutropG is elucidated as metabolism-oriented live-cell distinction (MOLD) through lipid droplet biogenesis with the help of ACSL and DGAT. Finally, NeutropG is applied to accurately quantify neutrophil levels in fresh blood samples by showing a high correlation with the current clinical method.
Author(s)
Gao Min이선혁박상혁권화영조희원정윤성장영태L. M. Ciaramicoli
Issued Date
2021
Type
Article
Keyword
cell recognitionfatty acidsfluorescent probesmetabolism
DOI
10.1002/anie.202108536
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8593
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_proquest_miscellaneous_2563423042&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Neutrophil-Selective%20Fluorescent%20Probe%20Development%20through%20Metabolism-Oriented%20Live-Cell%20Distinction&offset=0&pcAvailability=true
Publisher
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Location
독일
Language
영어
ISSN
1433-7851
Citation Volume
60
Citation Number
44
Citation Start Page
23743
Citation End Page
23749
Appears in Collections:
Medicine > Medicine
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