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Microparticle-tagged image-based cell counting (ImmunoSpin) for CD4+T cells

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Abstract
Affordable point-of-care (POC) CD4 + T lymphocyte counting techniques have been developed as alternatives to flow cytometry-based instruments caring for patients with human immunodeficiency virus (HIV)-1. However, POC CD4 enumeration technologies can be inaccurate. Here, we developed a microparticle-based visual detector of CD4 + T lymphocytes (ImmunoSpin) using microparticles conjugated with anti-CD4 antibodies, independent of microfluidic or fluorescence detection systems. Visual enumeration of CD4 + T cells under conventional light microscope was accurate compared to flow cytometry. Microparticle-tagged CD4 + T cells were well-recognized under a light microscope. ImmunoSpin showed very good precision (coefficients of variation of ImmunoSpin were <= 10%) and high correlation with clinical-grade flow cytometry for the enumeration of CD4 + T cells (y = 0.4232 + 0.9485 x for the %CD4 + T cell count, R-2 = 0.99). At thresholds of 200 and 350 cells/mu L, there was no misclassification of the ImmunoSpin system compared to the reference flow cytometry. ImmunoSpin showed clear differential classification of CD4 + T lymphocytes from granulocytes and monocytes. Because non-fluorescence microparticle-tags and cytospin slides are used in ImmunoSpin, they can be applied to an automatic digital image analyzer. Slide preparation allows long-term storage, no analysis time limitations, and image transfer in remote areas.
Author(s)
고대현박찬정성흥섭양정석오윤희오흥범장성수조영욱황상현
Issued Date
2021
Type
Article
Keyword
Human immunodeficiency virusImmunoSpinImage-based cell countingCD4+T cellMicroparticle
DOI
10.1007/s00604-021-05070-y
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8596
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_proquest_miscellaneous_2602636870&amp;context=PC&amp;vid=ULSAN&amp;lang=ko_KR&amp;search_scope=default_scope&amp;adaptor=primo_central_multiple_fe&amp;tab=default_tab&amp;query=any,contains,Microparticle-tagged%20image-based%20cell%20counting%20(ImmunoSpin)%20for%20CD4%2BT%20cells&amp;offset=0&amp;pcAvailability=true
Publisher
MICROCHIMICA ACTA
Location
오스트리아
Language
영어
ISSN
0026-3672
Citation Volume
188
Citation Number
12
Citation Start Page
0
Citation End Page
0
Appears in Collections:
Medicine > Medicine
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