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Determining the optimal administration conditions under which mif exerts neuroprotective effects by inducing bdnf expression and inhibiting apoptosis in an in vitro stroke model

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Abstract
Macrophage migration inhibitory factor (MIF) exerts neuroprotective effects against cerebral ischemia/reperfusion injury by inhibiting neuronal apoptosis and inducing the expression of brain-derived neurotrophic factor (BDNF). However, the optimal administration conditions of MIF are currently unknown. Here, we aimed to identify these conditions in an in vitro model. To deter-mine the optimal concentration of MIF, human neuroblastoma cells were assigned to one of seven groups: control, oxygen and glucose deprivation/reperfusion (OGD/R), and OGD/R with different concentrations (1, 10, 30, 60, and 100 ng/mL) of MIF. Six groups were studied to investigate the optimal administration time: control, OGD/R, and OGD/R with MIF administered at different times (pre-OGD, OGD-treat, post-OGD, and whole-processing). Water-soluble tetrazolium salt-1 assay, Western blot analysis, and immunocytochemistry were used to analyze cell viability and protein expression. We found that 60 ng/mL was the optimal concentration of MIF. However, the effects of administration time were not significant; MIF elicited similar neuroprotective effects regardless of administration time. These findings correlated with the expression of BDNF and apoptosis-related proteins. This study provides detailed information on MIF administration, which offers a foundation for future in vivo studies and translation into novel therapeutic strategies for ischemic stroke.
Author(s)
정철김미희김예영김지애고은재이승학김대열
Issued Date
2021
Type
Article
Keyword
apoptosisbrain-derived neurotrophic factorischemic strokemacrophage migration inhibitory factorneuroprotection
DOI
10.3390/brainsci11020280.
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8603
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=ULSAN_KORMARC2941877&context=L&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=Local%20Search%20Engine&tab=default_tab&query=any,contains,Determining%20the%20optimal%20administration%20conditions%20under%20which%20mif%20exerts%20neuroprotective%20effects%20by%20inducing%20bdnf%20expression%20and%20inhibiting%20apoptosis%20in%20an%20in%20vitro%20stroke%20model&offset=0&pcAvailability=true
Publisher
BRAIN SCIENCES
Location
스위스
Language
영어
ISSN
2076-3425
Citation Volume
11
Citation Number
2
Citation Start Page
1
Citation End Page
14
Appears in Collections:
Medicine > Medicine
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