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Preparation of human decellularized peripheral nerve allograft using amphoteric detergent and nuclease

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Abstract
Animal studies have shown that amphoteric detergent and nuclease (DNase I and ribonuclease A) is the most reliable decellularization method of the peripheral nerve. However, the optimal combination of chemical reagents for decellularization of human nerve allograft needs further investigation. To find the optimal protocol to remove the immunogenic cellular components of the nerve tissue and preserve the basal lamina and extracellular matrix and whether the optimal protocol can be applied to larger-diameter human peripheral nerves, in this study, we decellularized the median and sural nerves from the cadavers with two different methods: nonionic and anionic detergents (Triton X-100 and sodium deoxycholate) and amphoteric detergent and nuclease (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), deoxyribonuclease I, and ribonuclease A). All cellular components were successfully removed from the median and sural nerves by amphoteric detergent and nuclease. Not all cellular components were removed from the median nerve by nonionic and anionic detergent. Both median and sural nerves treated with amphoteric detergent and nuclease maintained a completely intact extracellular matrix. Treatment with nonionic and anionic detergent decreased collagen content in both median and sural nerves, while the amphoteric detergent and nuclease treatment did not reduce collagen content. In addition, a contact cytotoxicity assay revealed that the nerves decellularized by amphoteric detergent and nuclease was biocompatible. Strength failure testing demonstrated that the biomechanical properties of nerves decellularized with amphoteric detergent and nuclease were comparable to those of fresh controls. Decellularization with amphoteric detergent and nuclease better remove cellular components and better preserve extracellular matrix than decellularization with nonionic and anionic detergents, even in large-diameter human peripheral nerves. In Korea, cadaveric studies are not yet legally subject to Institutional Review Board review.
Author(s)
김재광배주열Shin Woo ChoiSuk Young ParkYoung Ho Shin
Issued Date
2021
Type
Article
Keyword
detergenthuman decellularized nerve graftmedian nervenucleasesural nerve
DOI
10.4103/1673-5374.306091
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8686
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_doaj_primary_oai_doaj_org_article_a6d99cc4293f42139dcbe93857ab1b35&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Preparation%20of%20human%20decellularized%20peripheral%20nerve%20allograft%20using%20amphoteric%20detergent%20and%20nuclease&offset=0&pcAvailability=true
Publisher
NEURAL REGENERATION RESEARCH
Location
중국
Language
영어
ISSN
1673-5374
Citation Volume
16
Citation Number
9
Citation Start Page
1890
Citation End Page
1890
Appears in Collections:
Medicine > Medicine
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