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Image correlation-based method to assess ciliary beat frequency in human airway organoids

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Alternative Title
Image correlation-based method to assess ciliary beat frequency in human airway organoids
Abstract
-----------e-pub(21.12.09 현재)--------------
Abstract? Ciliary movements within the human airway are essential for maintaining a clean lung environment.
Motile cilia have a characteristic ciliary beat frequency (CBF). However, CBF measurement with current video
microscopic techniques can be error-prone due to the use of the single-point Fourier transformation, which is often
biased for ciliary measurements. Herein, we describe a new video microscopy technique that harnesses a metric of
motion-contrast imaging and image correlation for CBF analysis. It can provide objective and selective CBF
measurements for individual motile cilia and generate CBF maps for the imaged area. The measurement performance
of our methodology was validated with in vitro human airway organoid models that simulated an actual human
airway epithelium. The CBF determined for the region of interest (ROI) was equal to that obtained with manual
counting. The signal redundancy problem of conventional methods was not observed. Moreover, the obtained CBF
measurements were robust to optical focal shifts, and exhibited spatial heterogeneity and temperature
dependence. This technique can be used to evaluate ciliary movement in respiratory tracts and determine whether it is
non-synchronous or aperiodic in patients. Therefore, our observations suggest that the proposed method can be
clinically adapted as a screening tool to diagnose ciliopathies.
Author(s)
최우준윤정기Bjorn Paulson이창훈임재준김종일김준기
Issued Date
2021
Type
Article
Keyword
Atmospheric modelingciliary beat frequencyciliary motionEstimationhuman airway organoidimage correlationImage resolutionLungMicroscopymotion-contrast imagingRedundacyTemperature measurement
DOI
10.1109/TMI.2021.3112992
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8936
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_ieee_primary_9539202&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Image%20correlation-based%20method%20to%20assess%20ciliary%20beat%20frequency%20in%20human%20airway%20organoids&offset=0&pcAvailability=true
Publisher
IEEE TRANSACTIONS ON MEDICAL IMAGING
Location
미국
Language
한국어
ISSN
0278-0062
Citation Volume
n
Citation Number
n
Citation Start Page
0
Citation End Page
0
Appears in Collections:
Engineering > Medical Engineering
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