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Characterizing Organelles in Live Stem Cells Using Label-Free Optical Diffraction Tomography

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Alternative Title
Characterizing Organelles in Live Stem Cells Using Label-Free Optical Diffraction Tomography
Abstract
Label-free optical diffraction tomography (ODT), an imaging technology that does not require fluorescent labeling or other pre-processing, can overcome the limitations of conventional cell imaging technologies, such as fluorescence and electron microscopy. In this study, we used ODT to characterize the cellular organelles of three different stem cells?namely, human liver derived stem cell, human umbilical cord matrix derived mesenchymal stem cell, and human induced pluripotent stem cell?based on their refractive index and volume of organelles. The physical property of each stem cell was compared with that of fibroblast. Based on our findings, the characteristic physical properties of specific stem cells can be quantitatively distinguished based on their refractive index and volume of cellular organelles. Altogether, the method employed herein could aid in the distinction of living stem cells from normal cells without the use of fluorescence or specific biomarkers.
Author(s)
김영규김태근신연희탁은영송기원오연목김준기백찬기
Issued Date
2021
Type
Article
Keyword
high density vesiclehuman stem celloptical diffraction tomographyorganelle volumethree-dimensional quantification
DOI
10.14348/molcells.2021.0190
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8937
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_9892007&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Characterizing%20Organelles%20in%20Live%20Stem%20Cells%20Using%20Label-Free%20Optical%20Diffraction%20Tomography&offset=0&pcAvailability=true
Publisher
MOLECULES AND CELLS
Location
대한민국
Language
영어
ISSN
1016-8478
Citation Volume
44
Citation Number
11
Citation Start Page
851
Citation End Page
860
Appears in Collections:
Engineering > Medical Engineering
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