Characterizing Organelles in Live Stem Cells Using Label-Free Optical Diffraction Tomography
- Alternative Title
- Characterizing Organelles in Live Stem Cells Using Label-Free Optical Diffraction Tomography
- Abstract
- Label-free optical diffraction tomography (ODT), an imaging technology that does not require fluorescent labeling or other pre-processing, can overcome the limitations of conventional cell imaging technologies, such as fluorescence and electron microscopy. In this study, we used ODT to characterize the cellular organelles of three different stem cells?namely, human liver derived stem cell, human umbilical cord matrix derived mesenchymal stem cell, and human induced pluripotent stem cell?based on their refractive index and volume of organelles. The physical property of each stem cell was compared with that of fibroblast. Based on our findings, the characteristic physical properties of specific stem cells can be quantitatively distinguished based on their refractive index and volume of cellular organelles. Altogether, the method employed herein could aid in the distinction of living stem cells from normal cells without the use of fluorescence or specific biomarkers.
- Author(s)
- 김영규; 김태근; 신연희; 탁은영; 송기원; 오연목; 김준기; 백찬기
- Issued Date
- 2021
- Type
- Article
- Keyword
- high density vesicle; human stem cell; optical diffraction tomography; organelle volume; three-dimensional quantification
- DOI
- 10.14348/molcells.2021.0190
- URI
- https://oak.ulsan.ac.kr/handle/2021.oak/8937
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_9892007&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Characterizing%20Organelles%20in%20Live%20Stem%20Cells%20Using%20Label-Free%20Optical%20Diffraction%20Tomography&offset=0&pcAvailability=true
- Publisher
- MOLECULES AND CELLS
- Location
- 대한민국
- Language
- 영어
- ISSN
- 1016-8478
- Citation Volume
- 44
- Citation Number
- 11
- Citation Start Page
- 851
- Citation End Page
- 860
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- Engineering > Medical Engineering
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