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Whole-cell display of Pyrococcus horikoshii glutamate decarboxylase in Escherichia coli for high-titer extracellular gamma-aminobutyric acid production

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Abstract
We investigated the effect of cell-surface display of glutamate decarboxylase (GadB) on gamma-aminobutyric acid (GABA) production in recombinant Escherichia coli. We integrated GadB from the hyperthermophilic, anaerobic archaeon Pyrococcus horikoshii to the C-terminus of the E. colt outer membrane protein C (OmpC). After 12 hr of culturing GadB-displaying cells, the GABA concentration in the extracellular medium increased to 3.2 el, which is eight times that obtained with cells expressing GadB in the cytosol. To further enhance GABA production, we increased the temperatures of the culture. At 60 degrees C, the obtained GABA concentration was 4.62 g/I after 12 hr of culture, and 5.35 g/l after 24 hr, which corresponds to a yield of 87.7%.
Author(s)
시바찬디란정재훈쿠마라벨 아쇼크 쿠마르홍순호
Issued Date
2021
Type
Article
DOI
10.1093/jimb/kuab039
URI
https://oak.ulsan.ac.kr/handle/2021.oak/9244
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_proquest_miscellaneous_2545990818&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Whole-cell%20display%20of%20Pyrococcus%20horikoshii%20glutamate%20decarboxylase%20in%20Escherichia%20coli%20for%20high-titer%20extracellular%20gamma-aminobutyric%20acid%20production&offset=0&pcAvailability=true
Publisher
JOURNAL OF INDUSTRIAL MICROBIOLOGY BIOTECHNOLOGY
Location
영국
Language
영어
ISSN
1367-5435
Citation Volume
48
Citation Number
7-8
Citation Start Page
039
Citation End Page
039
Appears in Collections:
Engineering > Chemical Engineering
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