KLI

Transcription Factor TonEBP Stimulates Hyperosmolality Dependent Arginine Vasopressin Gene Expression in the Mouse Hypothalamus

Metadata Downloads
Abstract
The hypothalamic neuroendocrine system is strongly implicated in body energy
homeostasis. In particular, the degree of production and release of arginine vasopressin
(AVP) in the hypothalamus is affected by plasma osmolality, and that hypothalamic AVP is
responsible for thirst and osmolality-dependent water and metabolic balance. However,
the osmolality-responsive intracellular mechanism within AVP cells that regulates AVP
synthesis is not clearly understood. Here, we report a role for tonicity-responsive enhancer
binding protein (TonEBP), a transcription factor sensitive to cellular tonicity, in regulating
osmosensitive hypothalamic AVP gene transcription. Our immunohistochemical work
shows that hypothalamic AVP cellular activity, as recognized by c-fos, was enhanced in
parallel with an elevation in TonEBP expression within AVP cells following water
deprivation. Interestingly, our in vitro investigations found a synchronized pattern of
TonEBP and AVP gene expression in response to osmotic stress. Those results
indicate a positive correlation between hypothalamic TonEBP and AVP production
during dehydration. Promoter and chromatin immunoprecipitation assays confirmed
that TonEBP can bind directly to conserved binding motifs in the 5’-flanking promoter
regions of the AVP gene. Furthermore, dehydration- and TonEBP-mediated hypothalamic
AVP gene activation was reduced in TonEBP haploinsufficiency mice, compared with wild
TonEBP homozygote animals. Therefore, our result support the idea that TonEBP is
directly necessary, at least in part, for the elevation of AVP transcription in dehydration
conditions. Additionally, dehydration-induced reductions in body weight were rescued in
TonEBP haploinsufficiency mice. Altogether, our results demonstrate an intracellular
machinery within hypothalamic AVP cells that is responsible for dehydration-induced
AVP synthesis.
Author(s)
김광곤김동희김진우박정우엄혜진이병주이태환정진권
Issued Date
2021
Type
Article
Keyword
TonEBPhyperosmolalityarginine vasopressinhypothalamusappetite behavior
DOI
10.3389/fendo.2021.627343
URI
https://oak.ulsan.ac.kr/handle/2021.oak/9564
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_doaj_primary_oai_doaj_org_article_6dc94839eeb6435f9ea993c47f93c6da&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Transcription%20Factor%20TonEBP%20Stimulates%20Hyperosmolality%20Dependent%20Arginine%20Vasopressin%20Gene%20Expression%20in%20the%20Mouse%20Hypothalamus&offset=0&pcAvailability=true
Publisher
Frontiers in Endocrinology
Location
스위스
Language
영어
ISSN
1664-2392
Citation Volume
12
Citation Number
1
Citation Start Page
627343
Citation End Page
627343
Appears in Collections:
Natural Science > Biological Sciences
Authorize & License
  • Authorize공개
Files in This Item:
  • There are no files associated with this item.

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.