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The Establishment of a Fast and Safe Orthotopic Colon Cancer Model Using a Tissue Adhesive Technique

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Abstract
Purpose We aimed to develop a novel method for orthotopic colon cancer model, using tissue adhesive in place of conventional surgical method. Materials and Methods RFP HCT 116 cell line were used to establish the colon cancer model. Fresh tumor tissue harvested from a subcutaneous injection was grafted into twenty nude mice, divided into group A (suture method) and group B (tissue adhesive method). For the group A, we fixed the tissue on the serosa layer of proximal colon by 8-0 surgical suture. For the group B, tissue adhesive (10 micro;L) was used to fix the tumor. The mortality, tumor implantation success, tumor metastasis, primary tumor size, and operation time were compared between the two groups. Dissected tumor tissue was analyzed for the histology and immunohistochemistry. Also, we performed tumor marker analysis. Results We observed 30% increase in graft success and 20% decrease in mortality, by using tissue adhesive method, respectively. The median colon tumor size was significantly increased by 4 mm and operation time was shortened by 6.5 minutes. The HE showed similar tumor structure between the two groups. The immunohistochemistry staining for cancer antigen 19-9, carcinoembryonic antigen, cytokeratin 20, and Ki-67 showed comparable intensities in both groups. Real-time quantitative reverse transcription analysis showed eight out of nine tumor markers are unchanged in the tissue adhesive group. Western blot indicated the tissue adhesive group expressed less p-JNK (apototic marker) and more p-MEK/p-p38 (proliferation marker) levels. Conclusion We concluded the tissue adhesive method is a quick and safe way to generate orthotopic, colon cancer model.
Author(s)
김건영박정훈윤성환이은지정재연Hong Tao HuLu-Shang JiangNader BakheetShi-Jun XuWang Zhe김명지송호영장수환
Issued Date
2021
Type
Article
Keyword
OrthotopicColon neoplasmsMouse modelTissue adhesive technique
DOI
10.4143/crt.2020.494
URI
https://oak.ulsan.ac.kr/handle/2021.oak/9575
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_9844201&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,The%20Establishment%20of%20a%20Fast%20and%20Safe%20Orthotopic%20Colon%20Cancer%20Model%20Using%20a%20Tissue%20Adhesive%20Technique&offset=0&pcAvailability=true
Publisher
CANCER RESEARCH AND TREATMENT
Location
대한민국
Language
영어
ISSN
1598-2998
Citation Volume
53
Citation Number
3
Citation Start Page
733
Citation End Page
743
Appears in Collections:
Natural Science > Biological Sciences
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