The Establishment of a Fast and Safe Orthotopic Colon Cancer Model Using a Tissue Adhesive Technique
- Abstract
- Purpose We aimed to develop a novel method for orthotopic colon cancer model, using tissue adhesive in place of conventional surgical method. Materials and Methods RFP HCT 116 cell line were used to establish the colon cancer model. Fresh tumor tissue harvested from a subcutaneous injection was grafted into twenty nude mice, divided into group A (suture method) and group B (tissue adhesive method). For the group A, we fixed the tissue on the serosa layer of proximal colon by 8-0 surgical suture. For the group B, tissue adhesive (10 micro;L) was used to fix the tumor. The mortality, tumor implantation success, tumor metastasis, primary tumor size, and operation time were compared between the two groups. Dissected tumor tissue was analyzed for the histology and immunohistochemistry. Also, we performed tumor marker analysis. Results We observed 30% increase in graft success and 20% decrease in mortality, by using tissue adhesive method, respectively. The median colon tumor size was significantly increased by 4 mm and operation time was shortened by 6.5 minutes. The HE showed similar tumor structure between the two groups. The immunohistochemistry staining for cancer antigen 19-9, carcinoembryonic antigen, cytokeratin 20, and Ki-67 showed comparable intensities in both groups. Real-time quantitative reverse transcription analysis showed eight out of nine tumor markers are unchanged in the tissue adhesive group. Western blot indicated the tissue adhesive group expressed less p-JNK (apototic marker) and more p-MEK/p-p38 (proliferation marker) levels. Conclusion We concluded the tissue adhesive method is a quick and safe way to generate orthotopic, colon cancer model.
- Author(s)
- 김건영; 박정훈; 윤성환; 이은지; 정재연; Hong Tao Hu; Lu-Shang Jiang; Nader Bakheet; Shi-Jun Xu; Wang Zhe; 김명지; 송호영; 장수환
- Issued Date
- 2021
- Type
- Article
- Keyword
- Orthotopic; Colon neoplasms; Mouse model; Tissue adhesive technique
- DOI
- 10.4143/crt.2020.494
- URI
- https://oak.ulsan.ac.kr/handle/2021.oak/9575
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- Publisher
- CANCER RESEARCH AND TREATMENT
- Location
- 대한민국
- Language
- 영어
- ISSN
- 1598-2998
- Citation Volume
- 53
- Citation Number
- 3
- Citation Start Page
- 733
- Citation End Page
- 743
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- Natural Science > Biological Sciences
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