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Transcriptome Analysis Identifies Altered Biological Processes and Novel Markers in Human Immunodeficiency Virus-1 Long-Term Non-Progressors

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Alternative Title
Transcriptome Analysis Identifies Altered Biological Processes and Novel Markers in Human Immunodeficiency Virus-1 Long-Term Non-Progressors
Abstract
Background: The latent reservoir of Human Immunodificiency Virus-1 (HIV-1) has been a
major barrier to the complete eradication of HIV-1 and the development of HIV therapy. Longterm
non-progressors (LTNPs) are a rare group of patients with HIV-1 who can spontaneously
control HIV-1 replication without antiretroviral therapy. Transcriptome analysis is necessary
to predict the pathways involved in the natural control of HIV-1, elucidate the mechanisms
involved in LTNPs, and find biomarkers for HIV-1 reservoir therapy.
Materials and Methods: In this study, we obtained peripheral blood mononuclear cells from
two LTNP subjects at multiple time points and performed RNA-sequencing analyses.
Results: We found that LTNPs and normal subjects had different transcriptome profiles.
Functional annotation analysis identified that differentially expressed genes in LTNPs
were enriched in several biological pathways such as cell cycle-related pathways and the
transforming growth factor-beta signaling pathway. However, genes that were downregulated
in LTNPs were associated with immune responses such as the interferon response and IL2-
STAT5 signaling. Protein-protein interaction network analysis showed that CD8A, KLRD1,
ASGR1, and MLKL, whose gene expression was upregulated in LTNPs, directly interacted
with HIV-1 proteins. The network analysis also found that viral proteins potentially regulated
host genes that were associated with immune system processes, metabolic processes, and
gene expression regulation.
Conclusion: Our longitudinal transcriptome analysis of the LTNPs identified multiple
previously undescribed pathways and genes that may be useful in the discovery of novel
therapeutic targets and biomarkers.
Keywords: Human immunodeficiency virus; HIV-1 non-progressors
Author(s)
김정은박지환윤철희이다연조영걸최병선최신영
Issued Date
2021
Type
Article
Keyword
Human immunodeficiency virus HIV-1 non-progressors RNA-seq Gene expression Transcriptome analysis
DOI
10.3947/ic.2021.0031
URI
https://oak.ulsan.ac.kr/handle/2021.oak/9582
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_9869892&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Transcriptome%20Analysis%20Identifies%20Altered%20Biological%20Processes%20and%20Novel%20Markers%20in%20Human%20Immunodeficiency%20Virus-1%20Long-Term%20Non-Progressors&offset=0&pcAvailability=true
Publisher
Infection & Chemotherapy
Location
대한민국
Language
영어
ISSN
2093-2340
Citation Volume
53
Citation Number
3
Citation Start Page
489
Citation End Page
502
Appears in Collections:
Natural Science > Biological Sciences
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