KLI

효모 유래 글루칸에 의한 HDAC5 유도 혈관신생성 활성화 연구

Metadata Downloads
Abstract
β-glucan is a polysaccharide found in large amounts in of bacteria, fungi, and yeast cell walls. The biological and physiological effects of β-glucan have been reported as anti-tumor, anti-diabetic, lowering cholesterol levels, and immunity enhancement. However, the role of yeast-derived β-glucan on angiogenesis has not been elucidated. There have been few specific studies on the clinical and physiological significance of yeast-derived β-glucan. Therefore, this study showed the correlation between β-glucan and histone deacetylase5 (HDAC5) in human umbilical vein endothelial cells (HUVECs), revealing the role of β-glucan on the activity of angiogenesis. We confirmed that HDAC5 was phosphorylated by β-glucan stimulation and released from the nucleus to the cytoplasm. In this study, we found that β-glucan-stimulated HDAC5 mediates the transcriptional activation of MEF2. As a result, the expression of KLF2, EGR2, and NR4A2 genes that are dependent on MEF2 and involved in angiogenesis increased. Thus, we showed the angiogenesis activity of β-glucan through cell migration, tube formation, and aortic ring analysis as in vitro and ex vivo angiogenesis assays. Specifically, inhibition of HDAC5 by an HDAC5 inhibitor repressed transcriptional activation of MEF2, in vitro and ex vivo angiogenesis. However, it was shown that β-glucan treatment restored MEF2 transcriptional activation, which had been inhibited. These findings suggest that HDAC5 is essential for angiogenesis and that β-glucan induces angiogenesis activity. In conclusion, this study demonstrates that β-glucan induces the activity of angiogenesis through HDAC5. It also suggests that β-glucan has potential value as a novel therapeutic agent modulating angiogenesis.
Author(s)
최민
Issued Date
2022
Awarded Date
2022-02
Type
dissertation
URI
https://oak.ulsan.ac.kr/handle/2021.oak/9875
http://ulsan.dcollection.net/common/orgView/200000594282
Affiliation
울산대학교
Department
일반대학원 의과학과
Advisor
하창훈
Degree
Master
Publisher
울산대학교 일반대학원 의과학과
Language
eng
Rights
울산대학교 논문은 저작권에 의해 보호 받습니다.
Appears in Collections:
Medical Science > 1. Theses (Master)
Authorize & License
  • Authorize공개
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.