The effect of FK506 (Tacrolimus) loaded with collagen membrane and fibrin glue on promotion of nerve regeneration in a rat sciatic nerve traction injury model

Abstract

Introduction In oral and maxillofacial surgery, peripheral nerve injury is one of the most common injuries that is likely to happen. The purpose of this study was to evaluate the effect of FK506 loaded with collagen membrane and fibrin glue to promote nerve regeneration after traction nerve injury in a rat model.

Material & Methods Thirty male Sprague-Dawley rats were divided into three groups. Group A (n=10) were sham group which included exposure of the sciatic nerve without any injury. Group B (n=10) and C (n=10) underwent traction nerve injury of 200 g for one minute. In group C, the injured nerve was covered with collagen membrane soaked with FK506 (0.5 mg/0.1 ml) and fibrin glue. Functional analysis and microscopic evaluation were performed at 2 and 4 weeks after injury.

Results The sciatic function index (SFI) was -5.78±3.07 for group A, -20.69±5.22 for group B, and -12.01±4.20 for group C, respectively in 2 week after injury. The SFI was -5.58±2.45 for group A, -19.69±4.81 for group B, and -11.95±1.94 for group C at 4 weeks. In both periods, significant differences were observed among each groups (p<0.017). Histomorphometric evaluation demonstrated improved nerve regeneration in group C compared to group B. However there were no statistical differences in axonal density among three groups (p<0.017).

Conclusion Localized FK506 with collagen membrane and fibrin glue could promote axonal regeneration in traction nerve injury of a rat model.