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Mechanistic Pathways for the Molecular Step Growth of Calcium Oxalate Monohydrate Crystal Revealed by In Situ Liquid-Phase Atomic Force Microscopy

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Abstract
Calcium oxalate monohydrate (COM) crystal is the most common crystalline component of human kidney stones. The molecular-scale inhibitory mechanisms of COM crystal growth by urinary biomolecules such as citrate and osteopontin adsorbed onto the crystal surface are now well understood. However, the pathways by which dissolved calcium and oxalate ions are incorporated into the molecular step of the COM crystal surface, leading to COM crystal growth. a prerequisite to be elucidated for developing effective therapeutics to inhibit COM stones.remain unknown. Here, using in situ liquid-phase atomic microscopy along with a step kinetic model, we reveal the pathways of the calcium and oxalate ions into the COM molecular step via the growth speed analysis of the molecular steps with respect to their step width at the nanoscale. Our results show that, primarily, the ions are adsorbed onto the terrace of the crystal surface from the solution.the rate-controlling stage for the molecular step growth, i.e., COM crystal growth.and then diffuse over it and are eventually incorporated into the steps. This primary pathway of the ions is unaffected by the model peptide D-Asp(6) adsorbed on the COM crystal surface, suggesting that urinary biomolecules will not alter the pathway. These new findings rendering an essential understanding of the fundamental growth mechanism of COM crystal at the nanoscale provide crucial insights beneficial to the development of effective therapeutics for COM kidney stones.
Author(s)
Kang Rae ChoJung-Hwan Lee서형석Yunseong JiJeung Hun ParkSang-Eui LeeHae-Won KimKuang Jen J. WuPrashant Kulshreshtha
Issued Date
2021
Type
Article
Keyword
calcium oxalate monohydrate crystalsin situ liquid-phase atomic force microscopystep kineticssurface diffusion crystal growth mechanismshuman kidney stones
DOI
10.1021/acsami.1c09245
URI
https://oak.ulsan.ac.kr/handle/2021.oak/8814
https://ulsan-primo.hosted.exlibrisgroup.com/primo-explore/fulldisplay?docid=TN_cdi_proquest_miscellaneous_2557232828&context=PC&vid=ULSAN&lang=ko_KR&search_scope=default_scope&adaptor=primo_central_multiple_fe&tab=default_tab&query=any,contains,Mechanistic%20Pathways%20for%20the%20Molecular%20Step%20Growth%20of%20Calcium%20Oxalate%20Monohydrate%20Crystal%20Revealed%20by%20In%20Situ%20Liquid-Phase%20Atomic%20Force%20Microscopy&offset=0&pcAvailability=true
Publisher
ACS APPLIED MATERIALS INTERFACES
Location
미국
Language
영어
ISSN
1944-8244
Citation Volume
13
Citation Number
31
Citation Start Page
37873
Citation End Page
37882
Appears in Collections:
Engineering > Aerospace Engineering
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